Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diversion of Arginine's dietary metabolic fate in proteinuric kidney disease.

Research square·2026
Same author

Kidney dysfunction is associated with mortality, adverse CT-based muscle metrics, and functional decline in surgically treated liposarcomas of the extremities and trunk.

PloS one·2026
Same author

Sequential Computed Tomography Morphometry-derived Muscle Loss Is Associated With Kidney Dysfunction and Mortality in Kidney Transplant Recipients Undergoing Serial Clinically Indicated Computed Tomography Imaging.

Transplantation direct·2026
Same author

FAS-controlled T cells drive lymphoproliferation through glycolysis without effector differentiation.

Journal of human immunity·2026
Same author

Intra-Individual Variability of Urinary EGF and Clusterin, and Effect of Frozen Storage on Stability: Results from UVALID.

International journal of molecular sciences·2026
Same author

Comprehensive Metabolite Profile and Cytotoxic Constituents of <i>Cryptolepis decidua</i>.

Journal of natural products·2026

Related Experiment Video

Updated: Jun 11, 2026

Reliable and High Efficiency Extraction of Kidney Immune Cells
09:15

Reliable and High Efficiency Extraction of Kidney Immune Cells

Published on: August 19, 2016

Optimized protocol for the multiomics processing of cryopreserved human kidney tissue.

Sydney E Gies1,2, Sonja Hänzelmann1,2,3,4, Dominik Kylies1,2

  • 1III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

American Journal of Physiology. Renal Physiology
|October 3, 2024
PubMed
Summary

Preservation media like RNAlater enable kidney biopsy biobanking for multi-omics analysis, including single-nucleus RNA sequencing and proteomics, advancing precision nephrology. Metabolomics requires snap-frozen tissue.

Keywords:
RNAlaterbiobankingkidney tissuemultiomicssingle-nucleus RNA sequencing

More Related Videos

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

Related Experiment Videos

Last Updated: Jun 11, 2026

Reliable and High Efficiency Extraction of Kidney Immune Cells
09:15

Reliable and High Efficiency Extraction of Kidney Immune Cells

Published on: August 19, 2016

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Published on: September 20, 2021

Area of Science:

  • Nephrology
  • Translational Medicine
  • Biobanking

Background:

  • Clinical kidney biopsies are crucial for research but require optimized biobanking protocols.
  • Current methods often necessitate liquid nitrogen, hindering integration into daily clinical workflows.
  • Multi-omic analyses demand tissue preservation methods compatible with various downstream applications.

Purpose of the Study:

  • To evaluate preservation media (RNAlater, CellCover) for kidney biopsy biobanking.
  • To assess compatibility with multi-omic techniques including single-nucleus RNA sequencing, proteomics, and metabolomics.
  • To establish protocols for integrating tissue biobanking into routine clinical practice for precision nephrology.

Main Methods:

  • Modified single-nucleus dissociation protocol for snap-frozen and preserved kidney tissue.
  • Single-nucleus RNA sequencing using Chromium 10X Genomics on porcine kidney tissue.
  • Assessment of preservation media for proteomics, metabolomics, and histopathology (including immunofluorescence).

Main Results:

  • RNAlater effectively preserves kidney tissue for single-nucleus RNA sequencing, proteomics, and histopathology.
  • Transcriptomic profiles showed minimal variation across storage conditions.
  • Metabolomic analysis was only feasible with snap-frozen tissue; other analyses were compatible with RNAlater.

Conclusions:

  • RNAlater is a suitable preservation medium for kidney biopsies in clinical settings, enabling multi-omic analyses.
  • This facilitates the creation of well-characterized biobanks for kidney disease research.
  • The developed protocol supports advancements in precision medicine for kidney diseases.