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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
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An absolute quantification method for selected MFGM proteins in infant formula using targeted proteomics.

Aikaterini Galani1, David A Goulding1, Jonathan O'Regan1

  • 1Nestlé Development Centre Nutrition, Wyeth Nutritionals Ireland, Askeaton, Co. Limerick, Ireland.

Food Chemistry
|October 3, 2024
PubMed
Summary
This summary is machine-generated.

This study developed a validated method to quantify four key milk fat globule membrane (MFGM) proteins in infant formula. This breakthrough aids in understanding MFGM protein health benefits, especially for infants.

Keywords:
Absolute quantificationInfant formulaMFGM proteinsMethod developmentTargeted proteomicsValidation

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Milk fat globule membrane (MFGM) proteins are recognized for their health benefits, particularly in infant nutrition.
  • Quantifying MFGM proteins is challenging due to their low abundance, complex interactions, and the intricate matrix of food products.
  • Accurate quantification is crucial for assessing the efficacy and quality of MFGM-containing products like infant formula.

Purpose of the Study:

  • To develop and validate an analytical protocol for the absolute quantification of specific MFGM proteins in infant formula.
  • To identify and quantify four key MFGM proteins: butyrophilin, mucin 1, xanthine dehydrogenase/oxidase, and perilipin 2.
  • To demonstrate the applicability of the developed method to MFGM ingredients.

Main Methods:

  • Selection of four target MFGM proteins relevant to infant formula.
  • Development of a robust analytical protocol for absolute quantification.
  • Validation of the method for accuracy, robustness, and low limit of quantification (LOQ).
  • Assessment of matrix effects in infant formula.

Main Results:

  • A validated analytical protocol for quantifying four specific MFGM proteins in infant formula was successfully established.
  • The method demonstrated high accuracy, robustness, and low LOQ, effectively managing matrix effects.
  • The protocol's feasibility was confirmed for analyzing MFGM ingredients.

Conclusions:

  • The developed analytical method provides a reliable tool for quantifying specific MFGM proteins in infant formula.
  • This advancement supports research into the health benefits of MFGM proteins, particularly for infant health.
  • The validated method contributes to improved quality control and characterization of infant nutrition products.