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Updated: May 2, 2026
Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
Published on: September 11, 2015
Harnessing molecular probes for imaging of human epidermal growth factor receptor (HER) family
Na Li1, Shengxi Chen2, Xiaoqing Cai1
1School of Pharmaceutical Sciences, Sun Yat-sen University, 132 East Outer Ring Road, Guangzhou 510006, China.
Abstract:
The human epidermal growth factor receptor (HER) family plays a critical role in the development, migration, and invasion of various cancers. Currently, the FDA has approved numerous targeting therapies for the HER family consist of small molecule drugs, monoclonal antibodies and antibody-drug conjugates. To facilitate precision therapy using currently approved targeted agents, early detection and quantification of each HER receptor are essential for assessment, treatment, and prognostic purposes. This study provides a comprehensive review of the latest advancements in detection and quantification of HER receptors, including traditional biopsies, liquid biopsies, and non-invasive detection methods. Although traditional histological methods, such as immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), have yielded valuable insights, advancements in real-time and non-invasive detection technologies necessitate improved methods for the dynamic evaluation of HER status. This article also reviews several emerging real-time techniques for detecting and quantifying HER status in circulating tumor cells (CTCs) extracted from blood samples, as well as in vivo assessments using positron emission tomography (PET) and single-photon emission computed tomography (SPECT) imaging. This review emphasizes the importance of continuous innovation in the application of HER receptor imaging technologies, with the goal of enhancing treatment outcomes and prognoses for cancer patients.
Insights
Accurate detection of human epidermal growth factor receptor (HER) family is crucial for cancer precision therapy. This review covers advancements in HER receptor detection, from traditional biopsies to novel non-invasive imaging techniques for better patient outcomes.
Area of Science:
- Oncology
- Biotechnology
- Medical Imaging
Background:
- The human epidermal growth factor receptor (HER) family is vital in cancer development and progression.
- FDA-approved targeted therapies exist for HER family, necessitating precise patient stratification.
- Accurate HER receptor assessment is key for effective cancer treatment and prognosis.
Purpose of the Study:
- To review recent advancements in HER receptor detection and quantification methods.
- To highlight the importance of dynamic HER status evaluation for precision therapy.
- To explore emerging real-time and non-invasive HER detection technologies.
Main Methods:
- Review of traditional biopsy methods (IHC, FISH).
- Exploration of liquid biopsy techniques for circulating tumor cells (CTCs).
- Assessment of in vivo imaging modalities (PET, SPECT).
Main Results:
- Traditional methods provide valuable insights but lack real-time dynamic evaluation.
- Liquid biopsies and advanced imaging offer non-invasive, real-time HER status monitoring.
- Emerging technologies enable dynamic quantification of HER receptors.
Conclusions:
- Continuous innovation in HER receptor imaging is essential for improving cancer treatment.
- Non-invasive and real-time detection methods enhance dynamic evaluation of HER status.
- Advanced HER detection technologies aim to improve patient outcomes and prognoses.
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