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Three-dimensional printed polyelectrolyte construct containing mupirocin-loaded quaternized chitosan nanoparticles
Yasir Qasim Almajidi1, Rana Kadum Muslim2, Anmar A Issa3
1Department of Pharmaceutics, College of Pharmacy, Al-Nahrain university, Baghdad, Iraq.
International Journal of Biological Macromolecules
|October 3, 2024
Summary
This study developed a 3D-printed hydrogel wound dressing using polyallylamine hydrochloride (PAH) and pectin (Pc) with mupirocin-loaded nanoparticles. The novel scaffold effectively prevents infection and accelerates skin healing in chronic wounds.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Nanotechnology
Background:
- Effective skin repair is hindered by recurrent wound infections.
- Advanced wound dressings integrating bioactive agents are needed.
- Three-dimensional (3D) printing offers a platform for complex scaffold fabrication.
Purpose of the Study:
- To fabricate a 3D-printed hydrogel scaffold incorporating mupirocin-loaded quaternized chitosan nanoparticles (QC NPs).
- To achieve controlled and sustained release of mupirocin (Mp) for enhanced wound treatment.
- To evaluate the scaffold's biocompatibility, antibacterial activity, and wound healing potential.
Main Methods:
- Fabrication of a 3D-printed hydrogel scaffold using polyallylamine hydrochloride (PAH) and pectin (Pc).
- Incorporation of mupirocin (Mp)-loaded quaternized chitosan nanoparticles (QC NPs) into the scaffold.
- Characterization of nanoparticle size, scaffold dimensions, hemolysis rate, antibacterial activity, cell viability, drug release kinetics, and in-vivo wound healing.
Main Results:
- QC-Mp nanoparticles measured 66.05 nm; scaffold dimensions were 147.22 ± 5.83 μm (strand) and 388.44 ± 14.50 μm (pore).
- Scaffolds exhibited low hemolysis (<2%), indicating good blood compatibility.
- The PAH-Pc/QC-Mp scaffold demonstrated significant antibacterial activity, enhanced HaCat cell viability, sustained Mp release (~60% by day 7), and promoted in-vivo wound healing.
Conclusions:
- The developed 3D-printed hydrogel scaffold is biocompatible and possesses antibacterial properties.
- The construct facilitates sustained mupirocin release, crucial for managing infected wounds.
- This innovative wound dressing shows significant potential for treating chronic and infected wounds by preventing infection and accelerating healing.
