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Combining Oligo Pools and Golden Gate Cloning to Create Protein Variant Libraries or Guide RNA Libraries for CRISPR

Alicia Maciá Valero1, Rianne C Prins1, Thijs de Vroet1

  • 1Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|October 3, 2024
PubMed

Keywords:
CRISPR librariesGolden Gate cloningMutational librariesOligo pools

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