Related Experiment Video

Updated: Jun 11, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.6K

Protocol for NT-CRISPR: A Method for Efficient Genome Engineering in Vibrio natriegens

Daniel Stukenberg1,2, Josef Hoff1,2,3, Anna Faber1,2,4

  • 1Center for Synthetic Microbiology, Philipps-Universität Marburg, Marburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|October 3, 2024
PubMed

Keywords:
BiotechnologyCRISPR-Cas9ChassisGenome engineeringGolden Gate cloningNatural transformationSynthetic biologyVibrio natriegens

More Related Videos

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination12:13

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Published on: October 8, 2012

14.8K
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

11.9K

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.9K
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.3K
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.8K