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  1. Home
  3. Downregulation Of Chemokine (c‑c Motif) Ligand 5 Induced By A Novel 8‑hydroxyquinoline Derivative (91b1) Suppresses Tumor Invasiveness In Esophageal Carcinoma.
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  3. Downregulation Of Chemokine (c‑c Motif) Ligand 5 Induced By A Novel 8‑hydroxyquinoline Derivative (91b1) Suppresses Tumor Invasiveness In Esophageal Carcinoma.

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Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses

Johnny Cheuk-On Tang1, Dessy Chan2, Po-Yee Chung2

  • 1Jean-Marie Lehn Laboratory, Guangzhou Huashang College, Guangzhou, Guangdong 511300, P.R. China.

International Journal of Molecular Medicine
|October 4, 2024

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
chemokine (C‑C motif) ligand 5esophageal squamous cell carcinomaquinoline derivatives

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A novel compound, 91b1, shows promise in treating esophageal squamous cell carcinoma (ESCC) by downregulating CCL5 expression. This mechanism effectively inhibits cancer cell proliferation and invasion, offering a potential new therapy for ESCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Drug Discovery

Background:

  • Esophageal squamous cell carcinoma (ESCC) is an aggressive cancer with high mortality.
  • Novel therapeutic strategies are needed to combat ESCC.
  • Understanding the molecular mechanisms underlying ESCC progression is crucial.

Purpose of the Study:

  • To investigate the anticancer activity of a novel 8-hydroxyquinoline derivative (91b1) in ESCC.
  • To elucidate the mechanisms by which 91b1 exerts its effects.
  • To evaluate 91b1 as a potential therapeutic agent for ESCC.

Main Methods:

  • In vitro cytotoxic effects evaluated using MTS assay across five ESCC cell lines.
  • Gene expression profiling using cDNA microarray, validated by qPCR and immunostaining.
  • Protein level analysis in archival ESCC samples and cell lines.

    • Main Results:

    • 91b1 demonstrated comparable anticancer effects to cisplatin.
    • Chemokine ligand 5 (CCL5) was identified as a significantly downregulated gene by 91b1.
    • CCL5 suppression occurred at both mRNA and protein levels in a dose-dependent manner.
    • Recombinant CCL5 enhanced ESCC cell invasion; CCL5 was upregulated in ESCC specimens.

    Conclusions:

    • 91b1 effectively inhibits ESCC cell proliferation and tumor invasion.
    • The anticancer effect of 91b1 is mediated through the downregulation of CCL5 expression.
    • 91b1 shows potential as a therapeutic agent for esophageal squamous cell carcinoma.