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Updated: Jun 11, 2025
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
Complete genome sequences of eight Pasteurella multocida isolates representing all lipopolysaccharide outer core loci
Amro Hashish1,2, Timothy J Johnson3, Mostafa Ghanem4
1Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
Abstract:
Pasteurella multocida (PM) is a major bacterial pathogen that causes fowl cholera disease in both domestic poultry and wild birds. Here, we report the complete genome sequences of eight PM isolates representing all known lipopolysaccharide outer core loci, which are phenotypically expressed as 16 known PM serotypes.
Insights
The complete genome sequences of eight Pasteurella multocida isolates were reported. These sequences represent all known outer core loci, explaining the 16 expressed serotypes of this poultry pathogen.
Area of Science:
- Bacteriology
- Genomics
- Avian Pathogens
Background:
- Pasteurella multocida (PM) is a significant bacterial pathogen responsible for fowl cholera.
- Fowl cholera affects both domestic poultry and wild bird populations.
- Understanding PM's genetic diversity is crucial for disease control.
Purpose of the Study:
- To sequence the complete genomes of eight Pasteurella multocida isolates.
- To characterize the lipopolysaccharide (LPS) outer core loci within these isolates.
- To correlate genomic data with the 16 known PM serotypes.
Main Methods:
- Whole-genome sequencing of eight PM isolates.
- Bioinformatic analysis of genomic data.
- Identification and characterization of LPS outer core loci.
Main Results:
- Complete genome sequences for eight PM isolates were successfully obtained.
- All known lipopolysaccharide outer core loci were represented across the sequenced isolates.
- The genomic data provides a basis for understanding the basis of the 16 expressed PM serotypes.
Conclusions:
- The reported genome sequences provide valuable genomic resources for Pasteurella multocida research.
- This study elucidates the genetic basis for the phenotypic diversity of PM serotypes.
- Findings can aid in the development of improved diagnostics and vaccines for fowl cholera.
