Recent advances in correlative cryo-light and electron microscopy
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Updated: Jun 11, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
Published on: June 24, 2013
Joshua A Pierson1, Jie E Yang2, Elizabeth R Wright3
1Department of Biochemistry, University of Wisconsin, Madison, WI, USA.
Abstract:
Correlative light and electron microscopy (CLEM) pipelines serve to integrate the imaging modalities of fluorescence light microscopy (FLM) and cryogenic electron microscopy (cryo-EM) to produce contextually relevant high-resolution structural snapshots of biological systems. Innovations in sample preparation, instrumentation, imaging, and data processing have advanced the field of cryo-EM. This review focuses on prior work and recent developments in the field of cryo- EM that support further integration of technologies for correlative microscopy workflows.
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