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SLMAP3 is essential for neurulation through mechanisms involving cytoskeletal elements, ABP, and PCP
Taha Rehmani1, Ana Paula Dias1, Billi Dawn Applin1
1Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Canada.
Life Science Alliance
|October 4, 2024
Summary
Loss of SLMAP3 protein disrupts neural tube development in mice by affecting cytoskeleton organization and planar cell polarity (PCP) pathways, leading to embryonic lethality. This highlights SLMAP3
Area of Science:
- Developmental biology
- Cell biology
- Neuroscience
Background:
- SLMAP3 is a tail-anchored membrane protein.
- SLMAP3 is implicated in Hippo signaling regulation.
- Global SLMAP3 loss causes embryonic lethality and neural tube defects.
Purpose of the Study:
- Investigate the role of SLMAP3 in neural tube development.
- Determine the molecular mechanisms by which SLMAP3 loss impacts neurulation.
- Clarify SLMAP3's relationship with Hippo signaling and planar cell polarity (PCP).
Main Methods:
- Analysis of SLMAP3 knockout mouse embryos.
- Assessment of neural plate morphology and convergent extension.
- Evaluation of planar cell polarity (PCP) and cytoskeletal protein expression and localization.
- Proteomic analysis to identify SLMAP3 interactors.
Main Results:
- SLMAP3 deficiency leads to arrested convergent extension and neural tube defects.
- Expression and activity of PCP components and downstream targets are dysregulated.
- Cytoskeletal and apical proteins are mislocalized in SLMAP3-deficient neural tubes.
- SLMAP3 interacts with cytoskeletal and PCP proteins.
Conclusions:
- SLMAP3 is essential for proper neural tube development in vivo.
- SLMAP3 regulates cytoskeleton organization and PCP pathway during neurulation.
- SLMAP3's role in neurulation is independent of Hippo signaling.
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