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Mouse Adipose Tissue Collection and Processing for RNA Analysis
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Transcriptome dataset of mouse adipose tissue across estrous cycles
Hongjie Zheng1, Yier Bai1, Shan Wu1
1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.
Scientific Data
|October 5, 2024
Summary
This study maps adipose tissue gene expression throughout the female mouse estrous cycle. These transcriptomic data reveal how reproductive hormones influence energy balance and metabolic health.
Area of Science:
- Reproductive Biology
- Metabolic Physiology
- Genomics
Background:
- Adipose tissue regulates energy homeostasis, and its dysfunction is linked to obesity and diabetes.
- The female reproductive cycle significantly impacts physiology, yet adipose tissue changes are not fully understood.
- Ovarian hormones exert regulatory control over adipose tissue, but comprehensive cycle-specific data are lacking.
Purpose of the Study:
- To comprehensively analyze the transcriptomic profile of female mouse adipose tissue across the entire estrous cycle.
- To provide a detailed resource for understanding estrous cycle-driven alterations in adipose tissue.
- To lay the groundwork for future research into the role of reproductive rhythmicity in adipose tissue function.
Main Methods:
- Transcriptomic profiling of female mouse adipose tissue.
- Analysis across all stages of the estrous cycle.
- Data processing, quality control, and validation.
Main Results:
- Detailed transcriptomic datasets of adipose tissue across the estrous cycle were generated.
- Data quality and robustness were confirmed through rigorous validation.
- Identification of gene expression patterns linked to reproductive cycle phases.
Conclusions:
- The generated dataset offers a valuable resource for studying estrous cycle influences on adipose tissue.
- This work enhances understanding of the interplay between reproductive status and metabolic health.
- Future research can leverage these findings to explore adipose tissue regulation by reproductive hormones.
