Related Experiment Video

Updated: Jun 11, 2025

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

7.4K

Helicobacter pylori PldA modulates TNFR1-mediated p38 signaling pathways to regulate macrophage responses for its

Wei Yang Sit1, Mei-Ling Cheng2, Tsan-Jan Chen1

  • 1Institute of Molecular and Cellular Biology & Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan, ROC.

Gut Microbes
|October 6, 2024
PubMed

Insights

Helicobacter pylori phospholipase A (PldA) disrupts macrophage immune responses, aiding bacterial survival and antibiotic resistance. Inhibiting PldA offers a promising therapeutic strategy against H. pylori infections.

Area of Science:

  • Microbiology
  • Immunology
  • Molecular Biology

Background:

  • Helicobacter pylori is a major cause of gastrointestinal diseases.
  • Increasing antibiotic resistance in H. pylori poses a significant clinical challenge.

Purpose of the Study:

  • To investigate the role of H. pylori phospholipase A (PldA) in host immune modulation.
  • To identify PldA as a potential therapeutic target against H. pylori.

Main Methods:

  • Studied the effect of PldA on macrophage membrane lipids and signaling pathways.
  • Assessed the impact of PldA inhibition on H. pylori viability and host cell survival.
  • Conducted in vivo studies to evaluate PldA's role in bacterial persistence and immune response.

Main Results:

  • PldA incorporates lysophosphatidylethanolamine into macrophage membranes, disrupting bilayer structure.
  • PldA impairs TNFR1-mediated p38-MK2 signaling, reducing autophagy and increasing apoptosis in macrophages.
  • Pharmacological inhibition of PldA reduced H. pylori viability and enhanced macrophage survival.
  • In vivo studies confirmed PldA's essential role in H. pylori persistence and immune cell recruitment.

Conclusions:

  • H. pylori PldA is critical for immune evasion by modulating host macrophage membranes via TNFR1 signaling.
  • PldA is a key virulence factor and a promising therapeutic target for combating H. pylori infections, including multidrug-resistant strains.
Keywords:
H. pylori phospholipase AHost-pathogen interactionTNFR1 signalinginnate immunitymembrane fluidity

More Related Videos

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection07:54

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Published on: September 2, 2014

46.9K
Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line06:38

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

27.3K

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.3K
Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors01:24

Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors

Peptic ulcer disease, commonly called PUD, represents a multifaceted condition characterized by disruptions in the lining of the gastrointestinal (GI)  tract. Central to the protection of the gastrointestinal lining is the mucosal-bicarbonate barrier. This physiological defense mechanism is a formidable shield against the corrosive effects of gastric acid and pepsin secretion in the stomach. Its role is pivotal in maintaining the structural integrity of the stomach's inner lining.
356
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
1.9K
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
959
Pathophysiology of Peptic Ulcer Disease: Injurious Factors01:22

Pathophysiology of Peptic Ulcer Disease: Injurious Factors

Peptic ulcers are sores on the stomach's inner lining and the upper small intestine, which are the result of disruptions in the mucosal layer that houses parietal cells which produce gastric acid, and chief cells which secrete pepsinogen.
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds...
542
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K