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Updated: Jun 11, 2025

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
Published on: November 17, 2023
TonEBP degradation is essential for microtubule nucleation and regrowth
Batchingis Chinbold1, Hyug Moo Kwon2, Raekil Park1
1Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, 61005, Republic of Korea.
Abstract:
TonEBP is a transcription factor known for its involvement in diverse physiological processes, including cell cycle, mitosis, migration, and cytoskeletal remodeling. However, the role of TonEBP regarding microtubules, essential structural components of the cytoskeleton, remains unclear. Here, we introduce a novel function for TonEBP as a regulator of microtubule nucleation. Our initial findings reveal that Nocodazole, a well-known microtubule depolymerizing agent, significantly downregulates the protein level of TonEBP. Moreover, microtubule depolymerization induces rapid degradation of TonEBP through the ubiquitin-proteasome pathway. Knockdown of TonEBP results in enhanced microtubule polymerization and regrowth, whereas the presence of TonEBP impairs microtubule nucleation. Collectively, our data suggest that TonEBP negatively regulates microtubule nucleation.
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