Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Research Domains
  5. Biological Sciences
  7. Plant Biology
  9. Phycology (incl. Marine Grasses)
  11. A Comparative Evaluation Of The Composition And Antioxidant Activity Of Free And Bound Polyphenols In Sugarcane Tips.
  1. Home
  3. Research Domains
  5. Biological Sciences
  7. Plant Biology
  9. Phycology (incl. Marine Grasses)
  11. A Comparative Evaluation Of The Composition And Antioxidant Activity Of Free And Bound Polyphenols In Sugarcane Tips.

Related Experiment Video

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
10:30

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

Published on: June 10, 2022

6.8K

A comparative evaluation of the composition and antioxidant activity of free and bound polyphenols in sugarcane tips.

Qiqi Huang1, Tongquan Wen1, Taowen Fang1

  • 1College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China.

Food Chemistry
|October 6, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Sugarcane tip bound polyphenols (SPB) significantly outweigh free polyphenols (SPF). SPB demonstrates superior antioxidant activity and enhances Caenorhabditis elegans health, indicating its potential as a natural antioxidant.

Keywords:
In vitro antioxidant activityIn vivo antioxidant activityMetabolite profilingPhenolic content

More Related Videos

Author Spotlight: Exploring Seaweed's Bioactive Compounds for Sustainable Innovations in Industries
10:18

Author Spotlight: Exploring Seaweed's Bioactive Compounds for Sustainable Innovations in Industries

Published on: November 21, 2023

2.5K
Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques
07:05

Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques

Published on: August 23, 2024

1.5K

Related Experiment Videos

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
10:30

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

Published on: June 10, 2022

6.8K
Author Spotlight: Exploring Seaweed's Bioactive Compounds for Sustainable Innovations in Industries
10:18

Author Spotlight: Exploring Seaweed's Bioactive Compounds for Sustainable Innovations in Industries

Published on: November 21, 2023

2.5K
Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques
07:05

Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques

Published on: August 23, 2024

1.5K

Area of Science:

  • Agricultural Chemistry
  • Biochemistry
  • Food Science

Background:

  • Sugarcane tips are rich in phenolic compounds.
  • Previous research focused on free polyphenols, neglecting bound polyphenols.
  • Bound polyphenols represent a largely unexplored source of bioactive compounds.

Purpose of the Study:

  • To quantify and characterize free and bound polyphenols in sugarcane tips.
  • To evaluate the antioxidant potential of bound polyphenols.
  • To investigate the in vivo effects of bound polyphenols on Caenorhabditis elegans.

Main Methods:

  • Quantification of free and bound polyphenols using spectrophotometry.
  • Identification and characterization of phenolic compounds via UPLC-EIS-QTOF-MS/MS.
Sugarcane tip
  • Assessment of antioxidant activity using ABTS+ and DPPH assays.
  • In vivo antioxidant assays using Caenorhabditis elegans.

    • Main Results:

    • Bound polyphenols (SPB) content (31.9 mg GAE/g DW) was significantly higher than free polyphenols (SPF) (3.4 mg GAE/g DW).
    • 44 free and 31 bound phenolics were identified, with SPB showing higher tannin content.
    • SPB exhibited stronger ABTS+ and DPPH radical scavenging activity.
    • SPB significantly improved Caenorhabditis elegans survival, antioxidant enzyme activity, and reduced ROS levels, comparable to Vitamin C at high concentrations.

    Conclusions:

    • Sugarcane tip bound polyphenols are more abundant and possess greater antioxidant capacity than free polyphenols.
    • Bound polyphenols from sugarcane tips show significant in vivo antioxidant effects.
    • Sugarcane tip bound polyphenols represent a promising source for natural antioxidant development.