Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Evidence for Evolution02:55

The Evidence for Evolution

42.5K
Genetic variations accumulating within populations over generations give rise to biological evolution. Evolutionary changes can result in the formation of novel varieties and entire new species. These changes are responsible for the diverse forms of life inhabiting the planet. The evidence for evolution suggests that all living organisms descended from common ancestors.
42.5K
Speciation Rates01:07

Speciation Rates

21.1K
Overview
21.1K
Genetic Drift03:33

Genetic Drift

39.5K
Natural selection—probably the most well-known evolutionary mechanism—increases the prevalence of traits that enhance survival and reproduction. However, evolution does not merely propagate favorable traits, nor does it always benefit populations.
39.5K
Excess Pressure Inside a Drop and a Bubble01:13

Excess Pressure Inside a Drop and a Bubble

1.6K
The shape of a small drop of liquid can be considered spherical, neglecting the effect of gravity. This drop can further be considered as two equal hemispherical drops put together due to surface tension. The forces acting on the spherical drop are due to the pressure of the liquid inside the drop, the pressure due to air outside the drop, and the force due to the surface tension acting on the two hemispherical drops.
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tomographic phenotypes and conditional time to atrophic conversion in dry age-related macular degeneration among progressors: an interval-censored study.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2026
Same author

Qualitative and Quantitative Assessment of Vitreous Inflammation in Uveitis: Current Limitations and Emerging Diagnostic Approaches.

Diagnostics (Basel, Switzerland)·2026
Same author

Case Report: Longitudinal analysis of local immunoregulatory mediators in a DLBCL vitreoretinal lymphoma receiving local chemotherapy.

Frontiers in medicine·2026
Same author

PK-SMART: Penetrating Keratoplasty With Sutureless Membrane Artificial Endothelial Replacement Technique. Report of the First Three Clinical Cases.

Cornea·2026
Same author

Response to letter to the editor: modified flapless surgical technique for sutureless scleral fixation of FIL SSF intraocular lens.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2026
Same author

Distinct Ocular Surface Microbial Profiles in Corneal Transplant Candidates.

Cornea·2026
Same journal

The Ocular Surface Consequences of Lacrimal Gland Loss Are Life-Stage Dependent.

The ocular surface·2026
Same journal

Evaluating synthetic anterior segment images for transferable eye disease recognition.

The ocular surface·2026
Same journal

Beyond diagnosis: AI-based surgical risk assessment in Acanthamoeba keratitis with real-world validation.

The ocular surface·2026
Same journal

Impact of the loss of tenascin X on tissue architecture and wound healing of the murine corneal stroma.

The ocular surface·2026
Same journal

Predisposing factors of infectious keratitis in chronic cicatrizing conjunctivitis.

The ocular surface·2026
Same journal

Topical application of low-concentration IL-2 enhances Treg's function and plays anti-inflammatory roles in experimental dry eye disease.

The ocular surface·2026
See all related articles

Related Experiment Video

Updated: Jun 11, 2025

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.7K

Big-bubble DALK: A technique in evolution.

Antonio Moramarco1, Lorenzo Gardini2, Ilaria Di Mola2

  • 1IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.

The Ocular Surface
|October 6, 2024
PubMed
Summary
This summary is machine-generated.

Deep anterior lamellar keratoplasty (DALK) using the big-bubble technique (BBT) offers a reproducible alternative to penetrating keratoplasty. This method enhances graft-host interface quality, improving outcomes for endothelial-sparing corneal diseases.

Keywords:
BB-DALKBig bubble techniqueCorneal transplantDALKDeep anterior lamellar keratoplastyPneumatic dissection

More Related Videos

Resurrection of Dormant Daphnia magna: Protocol and Applications
07:37

Resurrection of Dormant Daphnia magna: Protocol and Applications

Published on: January 19, 2018

18.3K
Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System
08:19

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System

Published on: May 9, 2021

2.1K

Related Experiment Videos

Last Updated: Jun 11, 2025

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.7K
Resurrection of Dormant Daphnia magna: Protocol and Applications
07:37

Resurrection of Dormant Daphnia magna: Protocol and Applications

Published on: January 19, 2018

18.3K
Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System
08:19

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System

Published on: May 9, 2021

2.1K

Area of Science:

  • Ophthalmology
  • Corneal Surgery
  • Transplantation

Background:

  • Deep anterior lamellar keratoplasty (DALK) is a preferred alternative to penetrating keratoplasty (PK) for corneal disorders not affecting the endothelium.
  • DALK preserves host endothelium, mitigating rejection risks and late graft failure.
  • Historically, DALK was challenging, limiting its widespread adoption.

Purpose of the Study:

  • To review the procedural steps, challenges, innovations, and complications of DALK utilizing the big-bubble technique (BBT).
  • To highlight the impact of understanding corneal anatomy, including Dua's layer, on BBT success.
  • To underscore BBT's role in improving DALK reproducibility and applicability.

Main Methods:

  • Review of procedural steps for DALK with BBT.
  • Analysis of factors influencing big bubble (BB) formation (e.g., pathology, trephination, air injection).
  • Discussion of technical innovations and complication management.

Main Results:

  • The big-bubble technique (BBT) significantly improves DALK reproducibility and reduces procedure time.
  • BBT facilitates separation of diseased stroma from healthy endothelium, creating a high-quality graft-host interface.
  • Clinical outcomes with BBT-enhanced DALK are comparable to PK.

Conclusions:

  • Understanding BB formation is crucial for surgical success in DALK.
  • Advancements in BBT have refined its efficacy and reproducibility for endothelial-sparing corneal transplantation.
  • DALK with BBT is a valuable, increasingly adopted technique for suitable corneal conditions.