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Updated: Aug 14, 2026
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A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
Published on: February 10, 2011
Rapid identification of Enterobacteriaceae by using noncommercial micro-tests in conjunction with API 20E profile
Journal of Clinical Microbiology
|October 1, 1979
Summary
This study identified 371 Enterobacteriaceae strains from 26 species using a cost-effective 96-well microdilution plate system. The API 20E profile data enabled rapid and accurate bacterial identification.
Area of Science:
- Microbiology
- Clinical Diagnostics
- Bacterial Identification
Background:
- Accurate identification of Enterobacteriaceae is crucial for clinical diagnostics and infection control.
- Traditional methods for bacterial identification can be time-consuming and expensive.
Purpose of the Study:
- To evaluate a rapid, accurate, and cost-effective method for identifying Enterobacteriaceae species.
- To assess the utility of a 96-well microdilution plate system combined with API 20E profile data.
Main Methods:
- A total of 382 Enterobacteriaceae strains, representing 26 species, were tested.
- Standard 96-well microdilution plates were utilized for the identification system.
- API 20E profile data was employed for analysis and comparison.
Main Results:
- 371 out of 382 strains (97% identification rate) were successfully identified.
- The method proved to be cheap, accurate, and rapid.
- The system effectively identified a broad range of Enterobacteriaceae species.
Conclusions:
- The 96-well microdilution plate system with API 20E data is a highly effective tool for Enterobacteriaceae identification.
- This method offers a practical and economical solution for clinical microbiology laboratories.
- The high identification rate supports its widespread adoption for routine bacterial analysis.
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