Related Experiment Video
Updated: May 9, 2026
06:18
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
14.2K
Dengue in Pune city, India (2017-2019): a comprehensive analysis.
Vidya Arankalle1, Shubham Shrivastava1, Ruta Kulkarni1
1Department of Communicable Diseases, Interactive Research School for Health Affairs, Bharati Vidyapeeth (Deemed to be University), Pune, India.
Frontiers in Public Health
|October 7, 2024
Summary
This study reveals that dengue diagnosis requires both NS1 and IgM tests for accuracy. Primary dengue infections can lead to severe symptoms, while secondary infections often present mildly, influenced by age and serostatus.
Area of Science:
- Infectious Diseases
- Epidemiology
- Virology
Background:
- Dengue remains a significant global health concern, with complex disease dynamics influenced by host and viral factors.
- Understanding the interplay of age, infection history (primary/secondary), and dengue virus (DENV) serotypes is crucial for effective disease management.
Purpose of the Study:
- To investigate the epidemiological dynamics of dengue disease in Pune City, India.
- To analyze the influence of age, primary/secondary infections, serostatus, and DENV serotypes on dengue presentation and severity over three years.
Main Methods:
- Conducted a 3-year study (2017-2019) in Pune City, India, diagnosing dengue using NS1/IgM ELISAs.
- Differentiated primary and secondary infections using IgG-capture ELISA.
- Determined DENV serotypes for 260 viral RNA-positive patients and recorded clinical symptoms.
Main Results:
- Over 44% of suspected cases were confirmed dengue; using NS1 or IgM alone would miss a significant proportion of cases.
- Secondary dengue infections were generally milder, whereas primary infections showed a higher tendency for warning signs.
- Dengue case numbers declined during the COVID-19 pandemic years, with yearly variations in DENV serotype prevalence.
Conclusions:
- Both NS1 and IgM testing are essential for efficient and comprehensive dengue diagnosis.
- Non-Antibody-Dependent Enhancement (ADE) mechanisms may contribute to severe dengue, highlighting the need for hospitalization in primary infections.
- Age and serostatus significantly impact dengue symptomology and disease progression.
Related Concept Videos
01:25Statistical Methods for Analyzing Epidemiological Data
Epidemiological data primarily involves information on specific populations' occurrence, distribution, and determinants of health and diseases. This data is crucial for understanding disease patterns and impacts, aiding public health decision-making and disease prevention strategies. The analysis of epidemiological data employs various statistical methods to interpret health-related data effectively. Here are some commonly used methods:
01:18Steps in Outbreak Investigation
In the ever-evolving field of public health, statistical analysis serves as a cornerstone for understanding and managing disease outbreaks. By leveraging various statistical tools, health professionals can predict potential outbreaks, analyze ongoing situations, and devise effective responses to mitigate impact. For that to happen, there are a few possible stages of the analysis:
01:26Principles of Disease Surveillance
Disease surveillance is the systematic collection, analysis, and interpretation of health data essential to the planning, implementation, and evaluation of public health practice. This process integrates data dissemination to entities responsible for preventing and controlling disease, injury, and disability. Surveillance systems provide crucial information for action, helping public health authorities make informed decisions to manage and prevent outbreaks, ensure public safety, optimize...
01:28Infectious Diseases and Their Occurrence
Infectious diseases appear in populations through various transmission patterns, influenced by pathogen characteristics, population immunity, environmental conditions, and social behavior. Understanding these patterns is essential for effective public health surveillance and intervention. These categories—sporadic, outbreak, epidemic, pandemic, and endemic—help frame the nature and scope of disease events.Sporadic diseases occur irregularly and infrequently, without a predictable temporal or...
01:23Investigation of Disease Outbreaks
Multistate foodborne outbreaks pose significant public health risks and require meticulous investigation to identify sources and implement control measures. The Centers for Disease Control and Prevention (CDC) utilizes a dynamic seven-step process for these investigations, integrating data from laboratories, interviews, and environmental assessments to protect public health.Outbreak Detection: The detection of multistate outbreaks typically begins with PulseNet, the CDC's national laboratory...
01:25Arboviral Encephalitis
Arboviral encephalitis refers to brain inflammation caused by arthropod-borne viruses, particularly those transmitted through mosquito vectors. Among these, West Nile virus (WNV), a member of the Flaviviridae family, is a significant public health concern. WNV is an enveloped, positive-sense, single-stranded RNA virus. Human infection typically begins when an infected mosquito introduces the virus into the dermis during feeding. The primary transmission cycle involves birds as amplifying hosts...