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Updated: Jun 11, 2025
Isolation of Adipose Tissue Immune Cells
Published on: May 22, 2013
ADIPOPHILIN PEPTIDE (ADPH 129-137) IS NOT A TARGET ANTIGEN FOR CD8+ T-CELLS IN PATIENTS WITH OBESITY
M Asadipour1, M R Ataollahi2, K Shams1
1Shiraz University of Medical Sciences, School of Medicine, Department of Immunology, Shiraz.
Context:
In obesity, the infiltration of leukocytes into adipose tissue seems to play a key role in the development of inflammation and insulin resistance. Over-expression of adipophilin (ADPH) in adipose tissue, a protein which regulates lipid droplet structure and formation, has been reported in some studies.
Objective:
To investigate the role of ADPH 129-137 as a target for CD8+ T-cells in PBMCs of patients with obesity.
Subjects And Methods:
PBMCs were obtained from 9 non-diabetic obese patients and 11 healthy subjects expressing the HLA-A0201 molecule. The ELISPOT assay used to monitor the presence of IFN-γ producing CD8+ T-cells against a HLA class I-restricted epitope derived from Adipophilin (ADPH 129-137) and two control peptides: Flu MP58-66 and Melan-A27-35.
Results:
The outcomes showed no significant difference between patient group and healthy donors in response to ADPH 129-137.
Conclusion:
These results demonstrated that ADPH 129-137 peptide possibly does not act as an autoantigen in patients with obesity.
