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Updated: Aug 16, 2026
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Identification of Protein Interacting Partners Using Tandem Affinity Purification
Published on: February 25, 2012
Immunoaffinity purification of tissue plasminogen activator from serum-supplemented conditioned media using
Thrombosis Research
|October 1, 1985
Abstract:
Tissue plasminogen activator (tPA) was purified from serum-supplemented culture media in a single-step immunoaffinity chromatographic procedure. This was done by producing a monoclonal antibody to tissue plasminogen activator. The antibody is a murine IgG1, kappa light chain molecule. The antibody was coupled to CNBr-activated Sepharose 4B. Tissue plasminogen activator was isolated from serum supplemented tissue culture media by immunoaffinity chromatography using a relatively high ionic strength equilibration buffer and an acidic elution buffer.
