Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

8.6K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
8.6K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.4K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

9.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
9.8K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heat-treatment-induced enhancement of activity and stability in Rh/Mo-doped PtNi octahedra for the oxygen reduction reaction.

Nanoscale·2025
Same author

Chemical termination and interfacial redox behavior of freestanding SrTiO<sub>3</sub>.

Scientific reports·2025
Same author

Terahertz Electronic and Spin Currents in Wafer-Scale Van der Waals Bi<sub>2</sub>Se<sub>3</sub>/WSe<sub>2</sub> Heterostructures and Polymorphs.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Demonstration of angular-momentum-resolved electron energy-loss spectroscopy.

Nature communications·2025
Same author

Intra-temporal and intracranial radiological abnormalities in cochlear implantation.

Irish medical journal·2025
Same author

Origin of giant enhancement of phase contrast in electron holography of modulation-doped n-type GaN.

Ultramicroscopy·2024

Related Experiment Video

Updated: Jun 11, 2025

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.7K

Increasing the Resolution of Transmission Electron Microscopy by Computational Ghost Imaging.

P Rosi1,2, L Viani2, E Rotunno1

  • 1<a href="https://ror.org/0042e5975">Institute of Nanosciences CNR-S3</a>, via G.Campi 213, 41125 Modena, Italy.

Physical Review Letters
|October 7, 2024
PubMed
Summary

Computational ghost imaging enhances transmission electron microscopy resolution beyond aberration limits. This technique uses structured illuminations and single-pixel detection for improved imaging, achieving a twofold resolution increase in simulations.

More Related Videos

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.3K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.1K

Related Experiment Videos

Last Updated: Jun 11, 2025

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.7K
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.3K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.1K

Area of Science:

  • Physics
  • Materials Science
  • Imaging Technology

Background:

  • Transmission electron microscopy (TEM) is crucial for nanoscale imaging.
  • Coherent aberrations limit the resolution of conventional TEM.
  • Overcoming these limitations is essential for advanced materials characterization.

Purpose of the Study:

  • To demonstrate computational ghost imaging (CGI) for high-resolution TEM.
  • To show CGI can surpass conventional resolution limits imposed by aberrations.
  • To validate the technique's potential under realistic experimental conditions.

Main Methods:

  • Numerical simulations of CGI applied to TEM.
  • Utilizing structured illuminations and single-pixel intensity measurements.
  • Optimizing probe design and illumination pattern coverage for enhanced performance.

Main Results:

  • Demonstrated CGI's capability to retrieve images in TEM.
  • Achieved a twofold increase in resolution beyond the aberration limit.
  • Showcased feasibility with a simple 8-electrode device example.

Conclusions:

  • Computational ghost imaging offers an innovative solution for high-resolution TEM.
  • The method effectively overcomes limitations from coherent aberrations.
  • This technique holds significant promise for future nanoscale imaging applications.