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Experimental Determination of Antibody Affinity and Avidity: Guidance and Considerations

Andrei Moroz1, Cory L Brooks2

  • 1Department of Clinical Analysis, Monoclonal Antibody Laboratory, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, Brazil.

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
|October 7, 2024
PubMed
Abstract

No abstract available in PubMed .

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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