Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional changes in marrow stromal cells in aplastic anaemia.

T Hotta, T Kato, H Maeda

    Acta Haematologica
    |January 1, 1985
    PubMed
    Summary

    Bone marrow stromal cells in aplastic anemia may have abnormalities affecting blood cell production. In some patients, this bone marrow microenvironment defect was reversible with androgen therapy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hydroxyl radical production by H2O2 plus Cu,Zn-superoxide dismutase reflects the activity of free copper released from the oxidatively damaged enzyme.

    The Journal of biological chemistry·1992
    Same author

    Effect of cilostazol, a cyclic AMP phosphodiesterase inhibitor, on the proliferation of rat aortic smooth muscle cells in culture.

    Journal of cardiovascular pharmacology·1992
    Same author

    Inhibition of mite protease (Df-protease) with protease inhibitors.

    Biochemistry international·1992
    Same author

    [Pharmacokinetic and clinical studies of cefprozil fine granules in children].

    The Japanese journal of antibiotics·1992
    Same author

    [A case of primary alveolar hypoventilation syndrome with a good response to nocturnal low-flow oxygen inhalation and negative pressure ventilation].

    Nihon Kyobu Shikkan Gakkai zasshi·1992
    Same author

    Microvasculature of normal and hydropic labyrinth.

    Scanning microscopy·1992

    Area of Science:

    • Hematology
    • Stem Cell Biology
    • Bone Marrow Microenvironment

    Background:

    • Aplastic anemia is a rare but serious blood disorder characterized by bone marrow failure.
    • The bone marrow microenvironment, including stromal cells, plays a critical role in supporting blood cell formation (hematopoiesis).
    • Previous studies suggest potential defects in the bone marrow microenvironment in aplastic anemia, but further investigation is needed.

    Purpose of the Study:

    • To investigate potential abnormalities in bone marrow stromal cells in patients with aplastic anemia.
    • To assess the capacity of marrow stromal cells to support hematopoiesis in a long-term culture system.
    • To explore the reversibility of any identified stromal dysfunction.

    Main Methods:

    • Utilized a long-term culture system to evaluate the supportive capacity of bone marrow stromal cells.

    Related Experiment Videos

  • Analyzed marrow stromal cell layers from patients diagnosed with aplastic anemia.
  • Monitored the maintenance of granulocyte-macrophage colony-forming cells in vitro.
  • Observed the effect of androgen therapy on stromal function in a subset of patients.

    • Main Results:

    • Marrow stromal cell layers from 3 out of 9 aplastic anemia patients demonstrated an impaired ability to maintain granulocyte-macrophage colony-forming cells in vitro.
    • This observed stromal dysfunction was reversible in one patient following treatment with androgen therapy.
    • These findings indicate a functional defect in the bone marrow microenvironment in a portion of aplastic anemia cases.

    Conclusions:

    • Bone marrow stromal cells can exhibit functional defects in aplastic anemia, impacting hematopoiesis.
    • The bone marrow microenvironment's role in aplastic anemia pathogenesis is significant.
    • Reversibility of stromal dysfunction with androgen therapy suggests potential therapeutic strategies targeting the microenvironment.