[Unimicon-s (universal microbiological control--sensitivity) standardized medium for determining the antibiotic...

A nutrient medium Unimicon-s for determination of antibiotic sensitivity of microorganisms is described. The medium is based on the raw material not used as a food, i. e. a clarified enzyme lysate of microbial biomass obtained at the All-Union Research Institute for Protein Synthesis. Unimicon-s provided satisfactory growth of various cultures and the sensitivity determination results analogous to those obtained with medium 1 (MPA) or Muller-Hinton medium recommended by the WHO as a standard medium for determination of microbial antibiotic sensitivity. The study on estimation of the fitness of the present criteria for dividing cultures by the sensitivity levels, i. e. into sensitive, moderate sensitive and resistant showed that with the use of an inoculation dose of 2.5 X 10(7) microbial cells per 1 ml the growth inhibition zones of the standard strains on the Unimicon-s medium were within the ranges of the permissible limits for medium 1. The Unimicon-s medium was tested with positive results in the laboratories of the A. N. Bakulev Institute of Cardiovascular Surgery and the 1st N. I. Pirogov City Clinical Hospital.