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Spore heat resistance and specific mineralization.

G R Bender, R E Marquis

    Applied and Environmental Microbiology
    |December 1, 1985
    PubMed
    Summary

    Demineralizing bacterial spores increases heat sensitivity, but remineralization, especially with calcium and manganese, restores resistance. Spore heat resistance is complexly linked to mineralization and killing temperature.

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    Area of Science:

    • Microbiology
    • Biophysics

    Background:

    • Bacterial spores exhibit remarkable resistance to heat, a property crucial for their survival.
    • The role of mineral content in spore heat resistance is not fully understood.

    Purpose of the Study:

    • To investigate the impact of demineralization and remineralization on the heat resistance of bacterial spores.
    • To elucidate the relationship between mineral composition and heat resistance across different temperatures.

    Main Methods:

    • Controlled acid titration was used to convert native bacterial spores (Bacillus megaterium, Bacillus subtilis niger, Bacillus stearothermophilus) into demineralized H forms.
    • Heat inactivation experiments were conducted on native, H, and remineralized spores.
    • Remineralization was achieved using various base solutions, followed by assessment of heat resistance.

    Main Results:

    • Demineralized (H) spores showed increased heat sensitivity but higher z-values compared to native spores.
    • The difference in heat sensitivity between native and H spores diminished at higher killing temperatures.
    • Remineralization, particularly with Ca²⁺ and Mn²⁺, restored heat resistance, with effectiveness varying by ion and temperature.
    • At higher temperatures, only a fraction of the mineral was effective in restoring resistance.

    Conclusions:

    • Mineralization is a critical factor in bacterial spore heat resistance.
    • The relationship between spore mineralization and heat resistance is complex and temperature-dependent.

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