[Effect of external factors on the kinetic properties of PGH-synthase]

The influence of external factors, viz. reaction mixture temperature, presence of low- and high-molecular (tween-20) organic compounds, urea and the solution high ionic strength on kinetic properties of PGH synthetase has been studied. Some factors (high ionic strength, addition of organic compounds) were found to slow down irreversible inactivation of PGH synthetase in the course of the reaction. Sensitivity of the enzyme to the change of the solution ionic strength depends on environmental factors, rising on the microsomal enzyme solubilization with tween-20 and decreasing on further enhancement of the tween-20 concentration.