Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Multiplexed, Image-based Pooled Screens In Primary Cells And Tissues With Perturbview.

Multiplexed, image-based pooled screens in primary cells and tissues with PerturbView.

Takamasa Kudo1, Ana M Meireles1, Reuben Moncada1

  • 1Genentech Research and Early Development, Genentech, Inc., South San Francisco, CA, USA.

Nature Biotechnology
|October 7, 2024

Related Experiment Videos

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.6K
Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
09:48

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

7.4K
Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
12:41

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Published on: December 23, 2022

4.8K

View abstract on PubMed

Summary
This summary is machine-generated.

PerturbView enhances optical pooled screening (OPS) by enabling highly multiplexed phenotypic readouts in diverse cell types and tissues. This new technology links cellular perturbations to complex phenotypes for broader biological discovery.

Area of Science:

  • Cell biology
  • Genomics

Related Experiment Videos

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.6K
Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
09:48

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

7.4K
Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
12:41

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Published on: December 23, 2022

4.8K
  • Biotechnology

    • Background:

    • Optical pooled screening (OPS) links image-based phenotypes to cellular perturbations.
    • Current OPS methods are limited to low-plex readouts in cancer cell lines due to in situ sequencing challenges.

    Purpose of the Study:

    • Develop an enhanced OPS technology (PerturbView) for highly multiplexed phenotypic readouts.
    • Expand OPS applications to diverse biological systems, including primary cells and tissues.
    • Enable in situ screens combining optical and transcriptomic phenotypes.

    Main Methods:

    • PerturbView utilizes in vitro transcription to amplify barcodes before in situ sequencing.
    • Demonstrated PerturbView in induced pluripotent stem cell-derived neurons, primary immune cells, and animal tumor tissue sections.
    • Combined PerturbView with spatial transcriptomics in mouse xenograft models.

    Main Results:

    • PerturbView enables highly multiplexed phenotypic readouts across diverse systems.
    • Screens in primary macrophages identified novel regulators of NF-κB signaling.
    • Integration with spatial transcriptomics allows for rich optical and transcriptomic phenotypes in situ.

    Conclusions:

    • PerturbView significantly advances OPS capabilities for complex biological systems.
    • The technology broadens the scope of OPS for various research models and applications.
    • PerturbView facilitates in situ screens with combined optical and transcriptomic data.