Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.1K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

5
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
5

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Realizing the potential of agonistic antibody immunotherapy.

Nature reviews. Drug discovery·2026
Same author

PD-1 signaling and PD-1 blockade-mediated tumor control are established at microvillar T cell contacts.

Science immunology·2026
Same author

Polymer Chemistry and the Kinetics of Nanoprecipitation.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Molecular Recognition-Driven Reaction-Based Sensing of Catecholamines in a Lipid Nanoreactor.

Journal of the American Chemical Society·2026
Same author

Digital defocus aberration interference for automated optical microscopy.

Nature communications·2026
Same author

A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Efficient evidence-based genome annotation with EviAnn.

Nature methods·2026
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
See all related articles

Related Experiment Video

Updated: Jun 11, 2025

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

10.7K

POLCAM: instant molecular orientation microscopy for the life sciences.

Ezra Bruggeman1,2, Oumeng Zhang3, Lisa-Maria Needham1,4

  • 1Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.

Nature Methods
|October 7, 2024
PubMed
Summary
This summary is machine-generated.

We developed POLCAM, a simplified single-molecule orientation localization microscopy (SMOLM) technique using a polarization camera. This method significantly speeds up molecular anisotropy determination and is easily adaptable for biological research.

More Related Videos

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

702
Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

9.9K

Related Experiment Videos

Last Updated: Jun 11, 2025

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

10.7K
Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

702
Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

9.9K

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Molecular Imaging

Background:

  • Current single-molecule orientation localization microscopy (SMOLM) methods are often slow and complex.
  • This complexity limits their widespread use in biological applications.

Purpose of the Study:

  • To develop a simplified and faster SMOLM method.
  • To enable easier adoption of SMOLM for biological research.

Main Methods:

  • Introduced POLCAM, a simplified SMOLM technique utilizing a polarization camera.
  • Developed theoretical frameworks to minimize field-of-view errors for polarization cameras.
  • Optimized experimental design using simulations.
  • Created a fast algorithm based on Stokes parameter estimation for rapid molecular anisotropy determination.

Main Results:

  • Achieved over 1,000-fold speed increase compared to existing methods for determining molecular anisotropy.
  • Demonstrated the ease of implementation on standard wide-field fluorescence microscopes.
  • Developed open-source software and resources to facilitate method adoption.

Conclusions:

  • POLCAM offers a significantly faster and simpler approach to SMOLM.
  • The method is readily applicable to various biological systems, including protein fibrils, cytoskeletons, and cell membranes.
  • POLCAM has the potential to broaden the accessibility and application of advanced molecular imaging techniques in life sciences.