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This study introduces a novel feed injection (FI) technique for ultrahigh-performance liquid chromatography, enabling detection of trace analytes at parts per billion (ppb) levels. This method significantly improves detection limits for challenging samples in dichloromethane.

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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC) faces challenges with strong injection solvents like dichloromethane (DCM).
  • Classic flow-through injection of DCM in aqueous mobile phases causes poor separation and peak splitting.
  • Achieving low parts per billion (ppb) detection limits is crucial for trace analysis.

Purpose of the Study:

  • To develop a robust method for low ppb level detection of analytes in DCM using RP-UHPLC.
  • To overcome the limitations of the strong solvent effect in chromatographic separations.
  • To demonstrate the efficacy of feed injection (FI) for trace analysis.

Main Methods:

  • Utilized reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC) with a core-shell stationary phase.
  • Employed a novel large-volume feed injection (FI) technique for injecting 45 μL of DCM solution.
  • Optimized feed speed and isocratic hold duration for separation of bisphenol A and its derivatives.

Main Results:

  • Feed injection (FI) achieved over a 20-fold improvement in the limit of detection (LOD) compared to standard injection.
  • Maintained excellent peak resolution despite the use of a large volume of strong solvent (DCM).
  • Established a method with LODs ranging from 1 to 10 ppb for analytes in polymer resin.

Conclusions:

  • Feed injection (FI) is a highly effective technique for trace analysis in challenging matrices where the strong solvent effect is prevalent.
  • This methodology offers significant potential for improving sensitivity in various analytical applications.
  • The developed method provides a reliable approach for quantifying trace contaminants in polymer resins.