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Updated: Jun 11, 2025

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
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Hsa-circ-ACSL1 Enhances Apoptosis and Autophagy in Myocarditis Cardiomyocytes Through the miR-7-5p/XBP1 Axis

Fu Li Liang1, You Fu Tong1, Xiao Chun Zhang1

  • 1Department of Coronary Heart Disease II, Qinghai Cardio-Cerebrovascular Specialty Hospital, Qinghai High Altitude Medical Research Institute, Xining City, Qinghai Province, China.

Anatolian Journal of Cardiology
|October 8, 2024
PubMed

Insights

Circular RNA ACSL1 (circ-ACSL1) exacerbates viral myocarditis (VMC) by increasing X-box binding protein 1 (XBP1) expression via microRNA-7-5p (miR-7-5p). circ-ACSL1 may serve as a diagnostic biomarker and therapeutic target for VMC.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • RNA Biology

Background:

  • Viral myocarditis (VMC) is a significant cardiovascular disease.
  • Circular RNAs (circRNAs) are implicated in cardiovascular disease pathophysiology.
  • The role of circRNAs in VMC is not well understood.

Purpose of the Study:

  • To investigate the biological functions of circ-ACSL1 in VMC.
  • To elucidate the regulatory mechanisms of circ-ACSL1 in VMC.

Main Methods:

  • VMC models were established using coxsackievirus B3 (CVB3) in mice and cell lines.
  • Inflammation, myocardial injury, apoptosis, and autophagy were assessed.
  • Expression levels of circ-ACSL1, microRNA-7-5p (miR-7-5p), and X-box binding protein 1 (XBP1) were manipulated.

Main Results:

  • Reducing circ-ACSL1 inhibited inflammation, autophagy, and apoptosis in VMC.
  • circ-ACSL1 targets miR-7-5p, regulating downstream XBP1.
  • Depleting miR-7-5p reversed the effects of circ-ACSL1 depletion.
  • Overexpressing circ-ACSL1 worsened VMC, an effect rescued by XBP1 knockdown.

Conclusions:

  • circ-ACSL1 promotes VMC by upregulating XBP1 through sponging miR-7-5p, leading to myocardial inflammation.
  • circ-ACSL1 shows potential as a biomarker for VMC diagnosis and a therapeutic target.
Abstract

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