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Lactoferrin modulates oxidative stress and inflammatory cytokines in a murine model of dysbiosis induced by clindamycin

  • 0Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire +

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October 8, 2024

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October 8, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Clindamycin-induced intestinal dysbiosis increases protein oxidation and inflammation. Bovine lactoferrin, particularly iron-saturated lactoferrin, effectively reversed these negative effects in mice, supporting gut health.

Area Of Science

  • Microbiology
  • Immunology
  • Biochemistry

Background

  • Antibiotics like clindamycin disrupt the gut microbiota, leading to dysbiosis, reduced anti-inflammatory properties, and altered immune responses.
  • Oxidative stress is a key consequence of antibiotic-induced intestinal damage.
  • Lactoferrin plays a role in maintaining intestinal homeostasis and mitigating antibiotic-induced dysbiosis.

Purpose Of The Study

  • To investigate the protective effects of native and iron-saturated bovine lactoferrin against clindamycin-induced intestinal dysbiosis in a murine model.
  • To assess the impact of lactoferrin on oxidative stress markers and inflammatory mediator expression in the ileum.

Main Methods

  • Male C57BL/6 mice were divided into six groups receiving saline, clindamycin, native lactoferrin (nLF), iron-saturated lactoferrin (sLF), nLF/clindamycin, or sLF/clindamycin.
  • Analysis of lipid peroxidation and protein carbonyl content in intestinal cells.
  • Quantitative real-time PCR (qRT-PCR) to determine the expression of inflammatory mediators like interleukin-6 and TNF-α.

Main Results

  • Clindamycin significantly increased protein carbonyl levels (approximately 5-fold) but did not alter lipid peroxidation.
  • Oral administration of iron-saturated lactoferrin (sLF) reversed the increase in protein carbonyls.
  • Clindamycin elevated interleukin-6 and TNF-α expression, an effect normalized by both native (nLF) and iron-saturated lactoferrin (sLF) treatments.

Conclusions

  • Bovine lactoferrin, especially the iron-saturated form, can counteract clindamycin-induced oxidative stress and inflammation in the gut.
  • Lactoferrin treatment helps restore intestinal homeostasis disrupted by antibiotic-induced dysbiosis.
  • These findings highlight lactoferrin's potential as a therapeutic agent to mitigate antibiotic side effects on the intestinal tract.

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