Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Diversity-in-a-dish: A practical framework for hiPSC model development.

Stem cell reports·2026

Same author

The ethical aspects of human organ-on-chip models: A mapping review.

Stem cell reports·2025

Same author

Promoting the adoption of best practices and standards to enhance quality and reproducibility of stem cell research.

Stem cell reports·2025

Same author

Mitochondrial fusion and cristae reorganization facilitate acquisition of cardiomyocyte identity during reprogramming of murine fibroblasts.

Cell reports·2025

Same author

Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction.

NPJ Regenerative medicine·2025

Same author

Spatial analysis of transcript and protein levels in skeletal muscle.

STAR protocols·2024

Same journal

Deep learning in tumour genomics: from multi-omics integration to precision oncology.

Open biology·2026

Same journal

Understanding GnRH: local systems, signalling mechanisms and implications in female health.

Open biology·2026

Same journal

The evolution and functional significance of neuropeptide cocktails: insights from SALMFamides in asteroid echinoderms.

Open biology·2026

Same journal

Structural basis of Drosophila insulin receptor activation by DILP2 hormone.

Open biology·2026

Same journal

Parental rearing shapes brain functional networks and socio-sexual behaviours in the prairie vole.

Open biology·2026

Same journal

The periosteum as an endocrine organ: historical foundations and new insights.

Open biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 11, 2025

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Published on: February 24, 2017

Cell cycle visualization tools to study cardiomyocyte proliferation in real-time.

Rustem Salmenov¹, Christine Mummery^1,2, Menno Ter Huurne¹

¹Department of Anatomy and Embryology, Leiden University Medical Center, Leiden 2300RC, The Netherlands.

|October 8, 2024

Summary

This summary is machine-generated.

Adult human heart cells do not regenerate after injury. This study reviews methods for tracking cardiomyocyte proliferation, aiming to discover new ways to regenerate heart tissue.

Keywords:

3D cardiac organoids cardiomyocyte proliferation human iPSC-derived cardiomyocytes live cell cycle reporter

More Related Videos

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

Published on: March 24, 2023

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

Related Experiment Videos

Last Updated: Jun 11, 2025

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Published on: February 24, 2017

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

Published on: March 24, 2023

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

Area of Science:

Cardiovascular Biology
Regenerative Medicine
Cell Cycle Regulation

Background:

Adult human cardiomyocytes are terminally differentiated and do not proliferate after injury, leading to limited heart regeneration.
Understanding cardiomyocyte cell cycle control is crucial for developing therapies to promote heart repair.
Current methods for quantifying cardiomyocyte proliferation in high-throughput screening are limited.

Purpose of the Study:

To review cardiomyocyte cell cycle characteristics.
To discuss fluorescent reporter systems for monitoring cell cycle progression.
To explore strategies for improving predictive value in high-throughput screening for cardiomyocyte proliferation.

Main Methods:

Review of existing literature on cardiomyocyte cell cycle and reporter systems.
Discussion of challenges in quantifying cardiomyocyte proliferation.
Consideration of advanced 3D human cardiac organoid platforms.

Main Results:

Conventional antibody staining for proliferation is costly and imprecise.
Live cell imaging offers high-throughput alternatives but has limitations.
Novel reporter systems and organoid models show promise for improved screening.

Conclusions:

Accurate quantification of cardiomyocyte proliferation is essential for drug discovery.
Advanced reporter systems combined with 3D organoids can identify pro-proliferative pathways.
This approach holds potential for stimulating human heart regeneration.