The O-GlcNAc database: introducing new features and tools developed from community feedback.

Area of Science:

• Biochemistry
• Molecular Biology
• Proteomics

Background:

• O-GlcNAc is a dynamic post-translational modification on serine/threonine residues of nucleocytoplasmic proteins.
• The O-GlcNAc Database (OSB) is a widely-used resource cataloging O-GlcNAcylated proteins, with hundreds of monthly users.

Purpose of the Study:

• To present an updated and expanded O-GlcNAc Database with enhanced features and broader scope.
• To incorporate community feedback for improved usability and data integration.

Main Methods:

• Expanded database content to include nearly 20,000 O-GlcNAcylated proteins and 48 species.
• Implemented new features: protein viewer mode, subcellular localization information, and an O-GlcNAc score.
• Integrated CytOVS tool for evaluating and sorting O-GlcNAcome datasets from MS experiments.

Main Results:

• The database has grown substantially in data volume and species coverage.
• New features enhance data visualization, analysis, and interpretation of O-GlcNAc modifications.
• Improved resource facilitates research in O-GlcNAc biology.

Conclusions:

• The updated O-GlcNAc Database is a significantly advanced and comprehensive resource for the scientific community.
• Enhanced features and expanded data provide greater utility for researchers studying O-GlcNAc biology.