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RNA-seq Based Transcriptome Analysis Reveals Role of Myoglobin in Rheumatoid Arthritis

Haibin Wang1, Xin Tian2, Le Ji2

  • 1Department of Orthopedic Surgery, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, China. wanghaibin19801211@163.com.

Inflammation
|October 8, 2024
PubMed

Insights

Gender influences rheumatoid arthritis (RA) risk. Myoglobin (MB) expression differs between sexes in healthy individuals and RA patients, suggesting a role in RA pathogenesis and sex-based disparities.

Area of Science:

  • Immunology
  • Genetics
  • Rheumatology

Background:

  • Rheumatoid arthritis (RA) is a chronic autoimmune disease causing joint destruction and bone erosion.
  • Current RA causes are unknown, and treatments have limitations and side effects.
  • RA affects women more than men, but the underlying sex-based mechanisms are poorly understood.

Purpose of the Study:

  • To investigate potential gender differences in rheumatoid arthritis (RA) prevalence.
  • To explore the molecular mechanisms underlying sex-based disparities in RA.

Main Methods:

  • Genome-wide transcriptome analysis of synovial biopsy samples.
  • Analysis of myoglobin (MB) expression in relation to sex and RA status.
  • Investigation of MB interactions with HLA-DMB and IL-6 in synovial cells (MH7A).

Main Results:

  • Myoglobin (MB) expression was significantly different between males and females.
  • Healthy males showed higher MB expression than females; RA patients exhibited the reverse pattern.
  • MB was found to interact with HLA-DMB and interleukin-6 (IL-6) in human synovial cells.

Conclusions:

  • Myoglobin (MB) exhibits sex-specific differential expression in rheumatoid arthritis (RA).
  • These findings suggest MB may play a role in the sex-based differences observed in RA prevalence and pathogenesis.
  • Further research into MB's interaction with HLA-DMB and IL-6 could reveal novel therapeutic targets for RA.
Keywords:
gene expressionhypoxiainflammatory cytokinesmyoglobinrheumatoid arthritissynovial tissue

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