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Related Experiment Video

Updated: Jun 11, 2025

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy
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Evaluation of urinary podocin and nephrin as markers of podocyturia in dogs with leishmaniosis.

Valeria Pantaleo1,2, Tommaso Furlanello1, Erika Carli1

  • 1San Marco Veterinary Clinic and Laboratory, Veggiano, Padua, Italy.

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|October 8, 2024
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Summary
This summary is machine-generated.

Urinary podocin and nephrin levels are lower in dogs with advanced leishmaniosis, indicating kidney damage. These markers help detect early renal injury in canine leishmaniosis.

Keywords:
Leishmania infantumCanineGlomerular diseaseRenal markers

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Area of Science:

  • Veterinary Medicine
  • Nephrology
  • Infectious Diseases

Background:

  • Renal disease is a primary cause of mortality in canine leishmaniosis.
  • Early detection of glomerular injury is crucial for preventing chronic kidney disease.
  • Podocyturia, indicated by urinary podocin and nephrin, can signal renal damage.

Purpose of the Study:

  • To assess urinary podocin and nephrin concentrations in dogs with leishmaniosis.
  • To evaluate these proteins as potential biomarkers for podocyturia and renal injury.
  • To correlate marker levels with the International Renal Interest Society (IRIS) staging of the disease.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to measure urinary podocin and nephrin.
  • Concentrations were normalized to urinary creatinine (uPoC and uNeC).
  • Dogs were categorized into healthy and leishmaniosis groups, with the latter staged using IRIS criteria.

Main Results:

  • Dogs with leishmaniosis exhibited lower urinary podocin and nephrin concentrations compared to healthy controls.
  • Healthy dogs and those in IRIS stage I had higher concentrations than dogs in advanced IRIS stages (II-IV).
  • No significant differences in uPoC and uNeC were found between healthy dogs and those with leishmaniosis across different IRIS stages.

Conclusions:

  • Lower urinary podocin and nephrin concentrations correlate with more severe kidney disease in advanced IRIS stages of canine leishmaniosis.
  • Urinary nephrin was detected in healthy dogs, suggesting its potential as a novel biomarker.
  • These findings highlight the utility of urinary podocin and nephrin in assessing renal health in dogs affected by leishmaniosis.