Related Experiment Video

Updated: Jun 11, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.6K

LncRNA PCBP1-AS1 suppresses cell growth in oral squamous cell carcinoma by targeting miR-34c-5p/ZFP36 axis

Orkideh Shafiee Allaf1, Wenhao Li2, Chongmai Zeng3

  • 1Department of Orthodontics, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510055, China. orkideh@163.com.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|October 9, 2024
PubMed

Insights

Long noncoding RNA PCBP1-AS1 is downregulated in oral squamous cell carcinoma (OSCC). PCBP1-AS1 suppresses OSCC cell growth by upregulating ZFP36 via interaction with miR-34c-5p.

Area of Science:

  • Molecular biology
  • Oncology
  • Genetics

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent and dangerous oral malignancy.
  • Bioinformatics analysis indicates long noncoding RNA PCBP1-AS1 is downregulated in OSCC.
  • Understanding the role of PCBP1-AS1 in OSCC is crucial for developing new therapeutic strategies.

Purpose of the Study:

  • To investigate the functions and molecular mechanisms of PCBP1-AS1 in OSCC.
  • To determine the expression levels of PCBP1-AS1 in OSCC cells.
  • To elucidate the regulatory pathway involving PCBP1-AS1, miR-34c-5p, and ZFP36 in OSCC.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) for gene expression analysis.
  • Cell viability, proliferation (CCK-8, colony-forming assays), and apoptosis (TUNEL, flow cytometry) assays.
  • RNA immunoprecipitation (RIP), RNA pulldown, and luciferase reporter assays to identify molecular interactions.

Main Results:

  • PCBP1-AS1 expression was found to be significantly downregulated in OSCC cells.
  • Overexpression of PCBP1-AS1 inhibited OSCC cell proliferation and induced apoptosis.
  • PCBP1-AS1 directly interacted with miR-34c-5p, negatively regulating its expression.
  • miR-34c-5p targeted the 3'untranslated region of ZFP36.
  • PCBP1-AS1 positively regulated ZFP36 expression, and ZFP36 silencing reversed the effects of PCBP1-AS1.

Conclusions:

  • PCBP1-AS1 acts as a tumor suppressor in OSCC.
  • PCBP1-AS1 inhibits OSCC cell growth by upregulating ZFP36 through the miR-34c-5p pathway.
  • This regulatory axis presents a potential therapeutic target for OSCC treatment.

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.6K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice00:07

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.2K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.7K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.2K