Plasma-derived Exosomal miR-25-3p and miR-23b-3p as Predictors of Response to Chemoradiotherapy in Esophageal Squamous Cell Carcinoma
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View abstract on PubMed
Summary
This summary is machine-generated.Exosomal miR-25-3p and miR-23b-3p show promise in predicting chemoradiotherapy response in esophageal cancer. MiR-25-3p also serves as a prognostic marker for progression-free survival in these patients.
Area Of Science
- Oncology
- Biomarker Discovery
- Molecular Diagnostics
Background
- Exosomal microRNAs (miRNAs) are emerging as crucial biomarkers in oncology.
- The predictive role of exosomal miRNAs in esophageal squamous cell carcinoma (ESCC) response to chemoradiotherapy (CRT) remains largely unexplored.
Purpose Of The Study
- To investigate the potential of exosomal miRNAs as predictive biomarkers for CRT response in ESCC patients.
- To identify specific exosomal miRNAs that can differentiate responders from non-responders to CRT.
Main Methods
- Prospective enrollment of 40 ESCC patients undergoing CRT.
- Plasma exosome isolation using the EXODUS platform.
- Small RNA sequencing for miRNA discovery in responders vs. non-responders, followed by qRT-PCR validation in an extended cohort.
Main Results
- Five differentially expressed exosomal miRNAs were identified in the discovery phase.
- Exosomal miR-23b-3p and miR-25-3p demonstrated significant ability to distinguish responders from non-responders (AUCs 0.708 and 0.932, respectively).
- Low miR-25-3p levels correlated with shorter progression-free survival (PFS), and miR-25-3p was identified as an independent predictive biomarker for PFS.
Conclusions
- Exosomal miR-25-3p and miR-23b-3p are promising biomarkers for predicting early CRT effectiveness in locally advanced ESCC.
- Exosomal miR-25-3p represents a novel prognostic marker for ESCC.
- Larger prospective studies are warranted to confirm their clinical utility for personalized treatment decisions.
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