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Selective Harvesting of Marginating-pulmonary Leukocytes
Published on: March 11, 2016
Changes in immune status of circulating NK cells in patients with latent tuberculosis infection
Shuang Qin1, Ruiqi Chen2, Meihui Li3
1Department of Clinical Laboratory, Jinan People's Hospital Affiliated to Shandong First Medical University, China.
Introduction:
The presence of patients with latent tuberculosis infection (LTBI) has fueled the tuberculosis pandemic. We aimed to investigate the immune status of NK cells in LTBI patients.
Material And Methods:
Twenty-one LTBI patients, 25 active pulmonary tuberculosis (APTB) patients and 25 healthy controls (HCs) participated in our research. The markers of NK cells were detected by flow cytometry.
Results:
The absolute number of circulating CD56bright and CD56dim NK cells in LTBI patients was higher than that of APTB patients, but the frequency of HLA-DR+ CD56bright NK cells was significantly lower than that of HCs and APTB patients. Also, LTBI patients with CD56bright NK cells had intracellular levels of granzyme B that were as significantly elevated as those with APTB patients, but the levels of granzyme A and perforin were reduced. Meanwhile, the frequencies of CXCR3+ NK cells, CXCR3+ CD56bright and CXCR3+ CD56dim NK cells were significantly lower in LTBI patients.
Conclusions:
Circulating CD56bright NK cells exerted a significant role in maintaining immune balance in LTBI patients. An elevated frequency of granzyme B+ CD56bright NK cells and a reduced frequency of perforin+ CD56bright NK cells were effective in differentiating LTBI patients from HCs.
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