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Published on: November 21, 2016
Transcriptome analysis of anaerobic glycolysis effects on Jurkat T cell proliferation
Ziyu Wang1, Hongyang Wang1, Qinghai Wang1
1The Affiliated Hospital of Qingdao University, China.
Introduction:
To explore the effects of anaerobic glycolysis on Jurkat T cell proliferation and clarify the possible mechanism via transcriptomic analysis.
Material And Methods:
The monocarboxylate transporter 1 inhibitor AZD3965 was used to target and block the transmembrane transport of lactate, thereby inhibiting anaerobic glycolysis in Jurkat T cells. Then, genes with differential expression between treated and untreated cells were detected by transcriptomic analysis, and constructs were generated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses as well as protein-protein interaction (PPI) network analysis were performed to explore the potential mechanism.
Results:
Inhibition of anaerobic glycolysis reduced Jurkat T-cell proliferation. RNA sequencing identified 1723 transcripts that were differentially expressed, including 1460 upregulated genes and 263 downregulated genes. GO functional enrichment analysis showed that the differentially expressed genes were mainly involved in the biological processes of response to unfolded protein, response to topologically incorrect protein, and protein folding. KEGG pathway analysis of differentially expressed genes or hub genes from the PPI network analysis revealed enrichment in the estrogen signaling and PI3K-Akt pathways.
Conclusions:
Anaerobic glycolysis contributes to the regulation of Jurkat T-cell proliferation. The underlying mechanism may involve the estrogen signaling pathway or PI3K-Akt signaling pathway as well as protein metabolism.
Insights
Inhibiting anaerobic glycolysis reduced Jurkat T-cell proliferation. Transcriptomic analysis revealed that this process involves the estrogen signaling and PI3K-Akt pathways, impacting protein metabolism.
Area of Science:
- Cell Biology
- Metabolic Regulation
- Immunology
Background:
- Anaerobic glycolysis is crucial for T cell function.
- Understanding its role in Jurkat T cell proliferation is essential.
Purpose of the Study:
- To investigate the impact of inhibiting anaerobic glycolysis on Jurkat T cell proliferation.
- To elucidate the molecular mechanisms underlying this effect using transcriptomic analysis.
Main Methods:
- Jurkat T cells were treated with AZD3965, a monocarboxylate transporter 1 inhibitor, to block anaerobic glycolysis.
- Transcriptomic analysis (RNA sequencing) was performed to identify differentially expressed genes.
- Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and protein-protein interaction (PPI) network analyses were conducted.
Main Results:
- Inhibition of anaerobic glycolysis significantly reduced Jurkat T-cell proliferation.
- RNA sequencing identified 1723 differentially expressed transcripts (1460 upregulated, 263 downregulated).
- Key affected biological processes included response to unfolded protein and protein folding. Enriched pathways were estrogen signaling and PI3K-Akt signaling.
Conclusions:
- Anaerobic glycolysis plays a regulatory role in Jurkat T-cell proliferation.
- The underlying mechanisms involve the estrogen signaling and PI3K-Akt signaling pathways, alongside protein metabolism.
- Targeting anaerobic glycolysis offers a potential therapeutic strategy for T cell-related conditions.
