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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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Extraction Free RT-PCR Surveillance Testing and Reporting for SARS-CoV-2.

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University of Wisconsin-Madison developed a rapid, inexpensive, and sensitive COVID-19 testing pipeline. This platform addresses early pandemic testing limitations and can be adapted for future disease surveillance.

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Area of Science:

  • Virology
  • Epidemiology
  • Public Health

Background:

  • The COVID-19 pandemic required widespread, rapid, and affordable testing for SARS-CoV-2 surveillance on university campuses.
  • Initial testing faced challenges including reagent shortages, low throughput, and delayed results.

Purpose of the Study:

  • To detail a SARS-CoV-2 testing pipeline established at the University of Wisconsin-Madison.
  • To highlight the platform's adaptability for other disease surveillance, variant testing, and future pandemics.

Main Methods:

  • Development of a comprehensive testing pipeline for SARS-CoV-2 surveillance.
  • Focus on achieving sensitivity, speed, and cost-effectiveness.

Main Results:

  • Established a functional testing pipeline addressing early pandemic limitations.
  • Demonstrated a platform capable of rapid, inexpensive, and sensitive SARS-CoV-2 surveillance.

Conclusions:

  • The developed testing pipeline effectively supported campus surveillance during the COVID-19 pandemic.
  • The platform's design offers a scalable model for future public health challenges and variant monitoring.