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Elp1 function in placode-derived neurons is critical for proper trigeminal ganglion development
Margaret A Hines1, Lisa A Taneyhill1
1Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland, USA.
Summary
Elongator acetyltransferase complex subunit 1 (Elp1) is crucial for trigeminal ganglion development in chicks. Its depletion impairs nerve size and innervation, offering insights into familial dysautonomia.
Area of Science:
- Developmental Neuroscience
- Molecular Biology
Background:
- The trigeminal nerve, the largest cranial nerve, originates from neural crest and placode cells.
- Molecular mechanisms of trigeminal ganglion development are poorly understood.
- Elongator acetyltransferase complex subunit 1 (Elp1) mutations cause familial dysautonomia (FD), characterized by sensory deficits.
Purpose of the Study:
- Investigate the role of Elp1 in trigeminal placode cells during gangliogenesis.
- Determine Elp1's function in the development of the trigeminal ganglion.
Main Methods:
- RNA sequencing of the developing chick trigeminal ganglion identified Elp1.
- Morpholino-mediated knockdown of Elp1 in chick trigeminal placode cells.
Main Results:
- Elp1 depletion significantly reduced trigeminal ganglion size.
- Aberrant innervation of the eye by placode-derived neurons was observed.
- Reduced axon outgrowth in trigeminal nerve branches was noted.
Conclusions:
- Elp1 plays a novel role in placode-derived neurons during trigeminal ganglion development.
- Findings provide insights into trigeminal ganglion development and FD etiology.
