Related Experiment Video
Updated: May 9, 2026
09:19
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
Published on: July 6, 2022
4.9K
Spatial Transcriptomics Unravel the Tissue Complexity of Oral Pathogenesis
J Haller1, N Abedi2, A Hafedi2
1Department of Periodontics, College of Dentistry, University of Illinois Chicago, Chicago, IL, USA.
Journal of Dental Research
|October 9, 2024
Summary
Spatial transcriptomics (ST) offers high-resolution gene expression analysis in tissues. This review highlights ST
Area of Science:
- Molecular Biology
- Genomics
- Histology
Background:
- Traditional transcriptomics lacks spatial resolution for complex tissue analysis.
- Understanding spatial gene expression is crucial for studying tissue development and disease.
Purpose of the Study:
- To review spatial transcriptomics (ST) workflows and applications.
- To showcase ST's utility in craniofacial development and disease pathogenesis.
Main Methods:
- Review of specimen processing, ST data analysis, and interpretation techniques.
- Application of visualization and cell deconvolution approaches for ST data.
Main Results:
- ST identified spatial gene patterns in craniofacial development (e.g., sutures, palate).
- ST revealed disease mechanisms in Sjögren's disease, head and neck cancers, and periodontal disease.
- ST uncovered specific cell subtypes and gene signatures in various pathologies.
Conclusions:
- Spatial transcriptomics provides unprecedented tissue-level resolution for biological insights.
- ST, combined with single-cell analysis, enhances understanding of craniofacial development and disease.
Related Concept Videos
01:27The Oral Microbiota
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
01:28Development of the Oral Microbiota
The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...