Related Experiment Video
Updated: Jun 11, 2025
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
Published on: October 28, 2016
Physicochemical Properties of IgD-type M Protein by Electrophoretic Analysis
Background:
We examined the physicochemical properties of IgD-type M protein from 10 patients with IgD-type M protein and those with other types of M protein.
Methods:
Identification of the L-chain type by routine methods (Immunoelectrophoresis: IEP, Immunofixation electrophoresis; IFE), detection of IgD-IgG complexes and the changes in the mobility by treatment with acid.
Results:
Identification of the L-chain type by both IEP and IFE was impossible. Only one patient revealed the possibility of IgD-IgG complex bands in 6 of the 10 samples. Changes in mobility by treatment with acid were observed in 8 of the 10 samples of IgD-type M protein.
Conclusions:
These abnormalities are caused by the primary structure of IgD, which may be related to the well-known weak reactivity with L-chain antibody.
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