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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Related Experiment Video

Updated: Jun 11, 2025

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
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CryoSeek: A strategy for bioentity discovery using cryoelectron microscopy.

Tongtong Wang1, Zhangqiang Li1, Kui Xu1

  • 1Beijing Frontier Research Center for Biological Structures, State Key Laboratory of Membrane Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.

Proceedings of the National Academy of Sciences of the United States of America
|October 9, 2024
PubMed
Summary
This summary is machine-generated.

Structural biology now discovers unknown biological entities using cryo-electron microscopy (cryo-EM). Researchers identified novel helical fibrils, TLP-1a and TLP-1b, potentially representing uncharacterized bacterial pili.

Keywords:
CryoNetCryoSeekdonor strand exchangefibrilsstructure-guided discovery

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Area of Science:

  • Structural Biology
  • Microbiology
  • Biophysics

Background:

  • Structural biology is shifting towards discovering novel bioentities.
  • Cryo-electron microscopy (cryo-EM) enables high-resolution structural determination.

Purpose of the Study:

  • To structurally characterize unknown helical fibrils from environmental water samples.
  • To investigate the assembly mechanism and potential biological relevance of these fibrils.

Main Methods:

  • Cryo-electron microscopy (cryo-EM) for structural analysis.
  • Bioinformatic analysis for protomer structure comparison.
  • Automodeling using CryoNet.

Main Results:

  • Two highly similar helical fibrils, TLP-1a and TLP-1b, were structurally determined.
  • Each fibril has an 8 nm diameter and a 15-Å wide tunnel.
  • The fibrils assemble via donor-strand exchange (DSE), a mechanism seen in bacterial pili.

Conclusions:

  • TLP-1a/b fibrils share structural similarities with bacterial pili, suggesting a potential link.
  • This study exemplifies the paradigm shift towards structure-guided discovery of unknown biological structures.