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Updated: Jun 11, 2025

A Microfluidic Platform to Study Bioclogging in Porous Media
Published on: October 13, 2022
A liquid static culture using a gas-permeable film bag contributes to microbiology
Kotaro Matsumoto1, Kazuya Higashi1, Yuki Naka1
1Graduate School of Biology-Oriented Science and Technology, Kindai University, 930 Nishimitani, Kinokawa, Wakayama, 649-6493, Japan.
Abstract:
We propose a simple tool for liquid static culture using a copolymer film with high gas permeability. The film bags were successfully used to culture microorganisms Escherichia coli, Komagataella phaffii (methylotrophic) and Bacillus sp. (biofilm-forming), with cells cultured under physical stress-free conditions with sufficient oxygen supply. Similar growth curves and plasmid productivity were observed for liquid shake and film bag E. coli cultures. The early growth response of the film bag culture following colony inoculation of liquid media differed from conventional shake cultures. Our results indicate that a gas-permeable film bag is a promising liquid culture tool and provides novel microbiology materials.
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