Transcriptomic changes in the PacC transcription factor deletion mutant of the plant pathogenic fungus Botrytis cinerea under acidic and neutral conditions
Related Experiment Video
Updated: Jun 11, 2025
Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates
Published on: July 2, 2018
Christine Rascle1, Bastien Malbert2, Isabelle Goncalves1
1Universite Claude Bernard Lyon 1, INSA Lyon, Bayer SAS, MAP, UMR5240, Villeurbanne, 69100, France.
Objectives:
Botrytis cinerea, the causal agent of gray mold, is a necrotrophic fungus that can infect a wide variety of plant species and plant tissues. During infection, this pathogen modulates the pH of its environment by secreting organic acids or ammonia. Deletion of the gene encoding the pH-responsive transcription factor PacC revealed the importance of this regulator in different steps of the infection process and particularly in the secretion of organics acids, reactive oxygen species and plant cell wall degrading enzymes. This study aimed to identify the genes controlled by this fungus-specific transcription factor when the fungus is placed under acidic or neutral conditions.
Data Description:
Botrytis cinerea B05.10 and the knock-out BcpacC mutant strains were grown on solid non-buffered medium for 3 days on the surface of cellophane membranes before transfer for 4 h onto the surface of liquid medium buffered at pH 5.0 or 7.0 followed by mycelium collection. After RNA sequencing, differentially expressed genes according to strains or pH conditions were listed. These data will be useful in understanding the adaptation process of B cinerea during plant infection.
Insights
Botrytis cinerea uses the PacC transcription factor to regulate genes involved in plant infection. This study identified PacC-controlled genes under acidic and neutral conditions, aiding understanding of fungal adaptation.
Area of Science:
- Plant Pathology
- Mycology
- Molecular Biology
Background:
- Botrytis cinerea causes gray mold disease in various plants.
- This fungus alters environmental pH during infection using organic acids and ammonia.
- The pH-responsive transcription factor PacC is crucial for B. cinerea infection processes.
Purpose of the Study:
- To identify genes regulated by the PacC transcription factor in Botrytis cinerea.
- To investigate PacC-controlled gene expression under acidic (pH 5.0) and neutral (pH 7.0) conditions.
Main Methods:
- Botrytis cinerea wild-type and BcpacC mutant strains were cultured.
- Mycelia were transferred to liquid media buffered at pH 5.0 or 7.0.
- Differential gene expression was analyzed using RNA sequencing.
Main Results:
- A list of differentially expressed genes was generated based on strain and pH.
- This data provides insights into PacC-mediated gene regulation.
- Understanding gene regulation is key to fungal adaptation during infection.
Conclusions:
- The study identified genes controlled by the PacC transcription factor in Botrytis cinerea.
- PacC plays a significant role in regulating gene expression in response to environmental pH.
- These findings contribute to understanding the molecular mechanisms of B. cinerea pathogenesis.
More Related Videos
11:16A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
Published on: July 22, 2017
08:03A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
Published on: March 28, 2017
Related Concept Videos
02:42Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
01:10Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...