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Nanozyme linked multi-array gas driven sensor for real-time quantitative detection of Group A streptococcus.

Qi Wang1, Pei Liu2, Ke Xiao3

  • 1Department of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. xiyun1993@163.com.

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A new nanozyme-linked sensor rapidly detects Group A Streptococcus (GAS) infections within two hours. This advanced diagnostic tool offers high sensitivity and accuracy for point-of-care testing, improving upon existing methods.

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Area of Science:

  • Biomedical Engineering
  • Diagnostic Technologies
  • Microbiology

Background:

  • Group A Streptococcus (GAS) causes millions of mild infections and hundreds of thousands of severe, potentially fatal invasive infections annually.
  • Rapid, accurate, and real-time detection of GAS is crucial for timely clinical intervention and public health management.
  • Existing diagnostic methods may lack the speed, sensitivity, or point-of-care accessibility required for effective GAS detection.

Purpose of the Study:

  • To develop a novel nanozyme-linked multi-array gas-driven sensor (NLMAGS) for rapid, point-of-care detection of Group A Streptococcus (GAS).
  • To evaluate the performance, sensitivity, and accuracy of the NLMAGS compared to established diagnostic techniques like ELISA and lateral flow immunoassays.

Main Methods:

  • Synthesized platinum- and palladium-rich nanozyme particles (Au@Pt@PdNPs) for labeling antibodies as detection probes.
  • Developed a double-antibody sandwich immunoassay using magnetic beads as capture probes and Au@Pt@PdNPs-labeled antibodies as detection probes.
  • Quantified GAS by measuring oxygen production, catalyzed by the immune complex, which drives ink drops forward in the gas-driven sensor.

Main Results:

  • The NLMAGS achieved a limit of detection (LOD) of 62 CFU mL⁻¹, which is 5 times lower than ELISA (334 CFU mL⁻¹).
  • Demonstrated excellent quantitative detection of GAS in spiked samples with high correlation to ELISA (r = 0.99, P < 0.001).
  • Outperformed traditional lateral flow immunoassays, showing a significantly lower LOD (62 CFU mL⁻¹ vs. 10⁴ CFU mL⁻¹).

Conclusions:

  • The NLMAGS provides a highly sensitive and rapid method for quantitative GAS detection at the point-of-care within 2 hours.
  • The sensor integrates nanozyme, immunoassay, and 3D printing techniques for enhanced performance and diagnostic capabilities.
  • The NLMAGS shows significant potential for detecting various pathogens beyond GAS, offering a versatile diagnostic platform.