Effect of copper on nitrogen uptake, transportation, assimilation processes, and related gene expression in Chinese cabbage [Brassica campestris L. ssp. Chinensis (L.)] under various nitrate-to-ammonium ratios
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Copper enhances Chinese cabbage growth by improving nitrogen metabolism. This essential trace element boosts enzyme activity and gene expression, positively impacting plant biomass and nutrient content.
Area Of Science
- Plant Nutrition
- Horticultural Science
- Plant Physiology
Background
- Copper (Cu) is vital for plant growth, but its role in nitrogen (N) metabolism, especially with varying nitrate/ammonium ratios, is poorly understood.
- Optimizing vegetable yield and quality relies on effective nutrient management.
Purpose Of The Study
- To investigate the effects of copper on nitrogen metabolism in Chinese cabbage under different nitrate/ammonium ratios.
- To elucidate the interaction between copper supply and nitrogen source on plant growth and N assimilation.
Main Methods
- Hydroponic cultivation of Chinese cabbage with two Cu concentrations (0 and 0.02 mg L⁻¹) and three nitrate/ammonium ratios (10/90, 50/50, 90/10).
- Measurement of plant biomass, enzyme activities (nitrate reductase, glutamine synthetase), gene expression (NIA, Gln2, NRT1.1, NRT2.1), and N content.
- Analysis of nitrate and free amino acid concentrations in shoots and roots.
Main Results
- Copper application increased biomass, nitrate reductase (NR) and glutamine synthetase (GS) activities, and N content in both shoots and roots.
- Cu influenced the expression of NR (NIA) and GS2 (Gln2) genes, and nitrate transporters (NRT1.1, NRT2.1).
- Cu treatment altered nitrate and free amino acid levels, with differential effects observed in shoots versus roots, modulated by nitrate/ammonium ratios.
Conclusions
- Copper plays a significant role in facilitating nitrate transport, enhancing nitrate reduction, and promoting ammonium assimilation in Chinese cabbage.
- Cu influences the transformation of organic nitrogen compounds, suggesting its potential to improve overall nitrogen metabolism.
- The effects of copper on nitrogen metabolism are significantly modulated by the supply ratio of nitrate to ammonium.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
The collective bacteria residing in and around plant roots are termed the rhizosphere. These soil-dwelling bacterial species are incredibly diverse....
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...