Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Anatomy of the Intestines01:23

Anatomy of the Intestines

71.5K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
71.5K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.5K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loss of WNT2B Increases Tumor Burden and Malignant Features in Colorectal Cancer.

Cancer communications (London, England)·2026
Same author

Temperature and developmental stage govern intestinal susceptibility to human coronavirus 229E.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

EGFR INHIBITION PROMOTES ENTEROENDOCRINE CELL DIFFERENTIATION CONTRIBUTING TO TREATMENT-ASSOCIATED DIARRHEA.

bioRxiv : the preprint server for biology·2026
Same author

Early-life mucosal T cells direct intestinal stem cell fate via a coordinated developmental program.

bioRxiv : the preprint server for biology·2026
Same author

Establishment of Zone-Enriched Primary Cultures from the Mouse Adrenal Cortex.

Journal of visualized experiments : JoVE·2026
Same author

Immune development in early life.

Nature immunology·2026

Related Experiment Video

Updated: Jun 11, 2025

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids
08:15

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Published on: November 15, 2019

6.0K

Cataloguing the postnatal small intestinal transcriptome during the first postnatal month.

Luiz Fernando Silva Oliveira1, Radhika S Khetani2, Yu-Syuan Wu1

  • 1Division of Newborn Medicine, Boston Children's Hospital, Boston, MA.

Biorxiv : the Preprint Server for Biology
|October 10, 2024
PubMed
Summary
This summary is machine-generated.

This study reveals key gene expression shifts in the developing mouse intestine during the first postnatal month. Understanding these changes in gene families offers insights into necrotizing enterocolitis (NEC) susceptibility and resistance.

Keywords:
adenosineimmunityintestinenecrotizing enterocolitisneonatal

More Related Videos

Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development
06:36

Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development

Published on: November 10, 2023

1.7K
Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

704

Related Experiment Videos

Last Updated: Jun 11, 2025

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids
08:15

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Published on: November 15, 2019

6.0K
Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development
06:36

Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development

Published on: November 10, 2023

1.7K
Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

704

Area of Science:

  • Developmental Biology
  • Genomics
  • Gastroenterology

Background:

  • The first postnatal month is critical for mouse intestinal maturation, transitioning from an immature to a mature state.
  • Necrotizing enterocolitis (NEC) is a significant concern in early intestinal development, with varying susceptibility and resistance periods.

Purpose of the Study:

  • To comprehensively survey gene expression changes in the developing mouse intestine (duodenum and ileum) from birth to 1 month of age using RNA-Seq.
  • To identify gene expression patterns correlating with periods of NEC susceptibility and resistance.
  • To validate key findings using complementary methods and compare them to human intestinal development.

Main Methods:

  • RNA-sequencing (RNA-Seq) of mouse intestines (C57Bl/6) from birth through 1 month of age.
  • Analysis of gene expression in duodenum and ileum to identify temporal changes.
  • Correlation analysis of gene families with NEC susceptibility and resistance.
  • Validation using quantitative reverse transcription PCR (qRT-PCR) and immunofluorescence.
  • Comparison with human intestinal biopsies and organoids.

Main Results:

  • Increased expression of DNA processing, vacuolar structure, tissue development, and cell migration genes correlated with NEC susceptibility.
  • Upregulation of immunity, intracellular transport, ATP production, and intracellular metabolism genes correlated with NEC resistance.
  • Key changes in cellular markers, epithelial regulators, immune cells, and adenosine metabolism were confirmed.

Conclusions:

  • Gene expression dynamics in the early postnatal mouse intestine are closely linked to NEC susceptibility and resistance.
  • This study provides a valuable reference dataset for understanding molecular roles in early intestinal development.
  • Findings offer potential insights into mechanisms underlying intestinal maturation and NEC pathogenesis.