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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

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Published on: June 17, 2020

Single-cell RNA sequencing and spatial transcriptomics of bladder Ewing sarcoma.

Weipu Mao¹, Kangjie Xu², Keyi Wang³

¹Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, China.

|October 10, 2024

Summary

This summary is machine-generated.

Researchers uncovered novel cell types and signaling pathways in bladder Ewing sarcoma/primitive neuroectodermal tumor (ES/PNET). Mast cells activate epithelial cells via TNFSF12-TNFRSF12A signaling, suggesting Enavatuzumab as a potential therapy.

Keywords:

cancer molecular biology transcriptomics

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Area of Science:

Oncology
Molecular Biology
Genomics

Background:

Bladder Ewing sarcoma/primitive neuroectodermal tumor (ES/PNET) is a rare, aggressive malignancy with poorly understood pathogenesis.
Existing treatments for bladder ES/PNET are limited, highlighting the need for novel therapeutic strategies.

Purpose of the Study:

To elucidate the underlying molecular mechanisms and cellular interactions driving bladder ES/PNET development.
To identify potential therapeutic targets for bladder ES/PNET.

Main Methods:

Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) were utilized to analyze tumor microenvironments.
Functional assays were performed to investigate cell-cell interactions and drug efficacy.

Main Results:

Identified unique epithelial (bladder ES-Epi) and mast cell (bladder ES-Mast) populations within bladder ES/PNET.
Discovered TNFSF12-TNFRSF12A ligand-receptor signaling mediating mast cell activation of epithelial cells.
Demonstrated Enavatuzumab's efficacy in inhibiting Ewing sarcoma cell line migration.

Conclusions:

Bladder ES/PNET pathogenesis involves specialized cell types and specific signaling pathways.
The TNFSF12-TNFRSF12A axis presents a promising therapeutic target for bladder ES/PNET.
Enavatuzumab shows potential as a novel treatment for this rare cancer.