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A carbon-based bifunctional heterogeneous enzyme: toward sustainable pollution control
Yuting Sun1, Ming Guo2, Shengnan Hu2
1College of Environmental and Resource Sciences, Zhejiang Agricultural & Forestry University Hangzhou Zhejiang 311300 China.
Chemical Science
|October 10, 2024
Summary
We developed a novel carbon-based heterogeneous enzyme for effective di(2-ethylhexyl)phthalate (DEHP) removal. This biomaterial demonstrates high selectivity and stability, offering a sustainable solution for organic pollutant remediation.
Area of Science:
- Biomaterials Science
- Environmental Chemistry
- Enzyme Engineering
Background:
- Conventional immobilized functional enzymes (IFEs) face challenges with carrier-induced degradation of enzyme activity.
- There is a need for next-generation IFEs with enhanced stability and functionality.
- Di(2-ethylhexyl)phthalate (DEHP) is a common plasticizer and environmental pollutant requiring efficient removal methods.
Purpose of the Study:
- To engineer a novel carbon-based bifunctional heterogeneous enzyme for intelligent recognition and removal of DEHP.
- To investigate the dual-response performance (enrichment and degradation) of the engineered enzyme.
- To analyze the enzyme's kinetic behavior and stability under various conditions.
Main Methods:
- Fabrication of a molecularly imprinted polymer (MIP) on multi-walled carbon nanotubes (MWCNTs) functionalized with lipase.
- Investigating the enrichment and degradation capabilities of the MIP-AMWCNTs@lipase composite for DEHP.
- Evaluating the imprinting factor, removal rate, catalytic efficiency, and operational stability of the heterogeneous enzyme.
- Developing a stepwise heterogeneous enzyme reaction kinetic model based on Michaelis-Menten kinetics.
Main Results:
- The engineered MIP-AMWCNTs@lipase demonstrated selective enrichment of DEHP with an imprinting factor of 3.4.
- Achieved a DEHP removal rate of up to 94.2% within a short timeframe.
- The heterogeneous enzyme exhibited robust activity, catalytic efficiency, and retained 77.7% of its initial activity after 7 cycles under harsh conditions.
Conclusions:
- The developed carbon-based bifunctional heterogeneous enzyme offers a promising strategy for the selective removal of organic pollutants like DEHP.
- The study highlights the potential of combining molecular imprinting and enzyme immobilization for advanced biomaterial design.
- Findings contribute to the advancement of biomaterials and environmental remediation technologies.
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